Method for producing tridecanedioic acid by candida viswanathii fermentation
A technology of thirteen carbon dibasic acid and Candida viesis, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of long production equipment time, low fermentation level, and inability to solve binary Acid industrialization problems and other issues
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Embodiment 1
[0033] The process of fermentative synthesis of long-chain dibasic acids is as follows:
[0034] 1. Culture medium preparation
[0035] ① Incline medium: malt juice agar medium;
[0036] ②Seed medium: glucose 25g / L, disodium hydrogen phosphate 6g / L, yeast extract 3g / L, corn steep liquor 5g / L, urea 1.5g / L, NaCl 0.5g / L, KCl 1g / L, Tween 0.5 g / L.
[0037] ③ Fermentation medium: glucose 40g / L, disodium hydrogen phosphate 8g / L, yeast extract 3g / L, corn steep liquor 5g / L, urea 1.5g / L, NaCl 0.5g / L, KCl 1g / L, Tween 0.5 g / L.
[0038] 2. Seed cultivation stage
[0039] A piece of Candida viswanathii ws-1301 was spread on a solid slant medium in a large test tube of 20×180 mm, and cultured at 27° C. for 72 hours. Inoculate the activated seed culture on the slant into a 500ml Erlenmeyer flask containing 200ml of seed medium, shake and cultivate at 27°C and 250r / min for 48 hours, and the OD of the detected strain is between 0.6 and 0.85.
[0040] 3. Fermentation acid production stage:...
Embodiment 2
[0044] The process of fermentative synthesis of long-chain dibasic acids is as follows:
[0045] 1. Culture medium preparation
[0046] ① Incline medium: malt juice agar medium;
[0047] ②Seed medium: glucose 25g / L, disodium hydrogen phosphate 6g / L, yeast extract 3g / L, corn steep liquor 5g / L, urea 1.5g / L, NaCl 0.5g / L, KCl 1g / L, Tween 0.5 g / L.
[0048] ③ Fermentation medium: glucose 35g / L, disodium hydrogen phosphate 8g / L, yeast extract 3g / L, corn steep liquor 5g / L, urea 1.5g / L, NaCl 0.5g / L, KCl 1g / L, Tween 1.5 g / L.
[0049] 2. Seed cultivation stage
[0050] A piece of Candida viswanathii ws-1301 was spread on a solid slant medium in a large test tube of 20×180 mm, and cultured at 27° C. for 72 hours. Inoculate the activated seed culture on the slant into a 500ml Erlenmeyer flask containing 200ml of seed medium, and culture it with shaking at 27°C and 250r / min for 48 hours. In a base 10L fermenter, the initial pH is 6.0, the temperature is 27°C, the ventilation rate is 1...
Embodiment 3
[0055] The process of fermentative synthesis of long-chain dibasic acids is as follows:
[0056] 1. Culture medium preparation
[0057] ① Incline medium: malt juice agar medium;
[0058] ②Seed medium: glucose 25g / L, disodium hydrogen phosphate 6g / L, yeast extract 3g / L, corn steep liquor 5g / L, urea 1.5g / L, NaCl 0.5g / L, KCl 1g / L, Tween 0.5 g / L.
[0059] ③Fermentation medium: glucose 35g / L, disodium hydrogen phosphate 8g / L, yeast extract 3g / L, corn steep liquor 5g / L, urea 1.5g / L, NaCl 0.5g / L, KCl 1g / L, Tween 1.5 g / L.
[0060] 2. Seed cultivation stage
[0061] A piece of Candida viswanathii ws-1301 was spread on a solid slant medium in a large test tube of 20×180 mm, and cultured at 27° C. for 72 hours. Inoculate the activated seed culture on the slant into a 500ml Erlenmeyer flask containing 200ml of seed medium, and culture it with shaking at 27°C and 250r / min for 48 hours. In a base 5L fermenter, the initial pH is 6.0, the temperature is 27°C, the ventilation rate is 1:0...
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