Quality control material for metabolomics detection and quality control method of quality control material

A technology of metabolomics and quality control methods, applied in the field of quality control products for metabolomics detection and its quality control, can solve problems such as instrument pollution, cumbersome operation, and overlapping detection peaks, and achieve low cost, low dosage, Formulate simple effects

A technology of metabolomics and quality control methods, applied in the field of quality control products for metabolomics detection and its quality control, can solve problems such as instrument pollution, cumbersome operation, and overlapping detection peaks, and achieve low cost, low dosage, Formulate simple effects

CN110618215AActive Publication Date: 2019-12-27嘉兴迈维代谢生物科技有限公司
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  • Quality control material for metabolomics detection and quality control method of quality control material
  • Quality control material for metabolomics detection and quality control method of quality control material
  • Quality control material for metabolomics detection and quality control method of quality control material

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Embodiment 1

[0025] The invention adopts ultra-high performance liquid chromatography-triple quadrupole / linear ion trap tandem mass spectrometry (UPLC-QTRAP) technology, the mass spectrometer is AB Sciex Q-TRAP 6500, and the analysis software is Multi Quant. The control product is made up of 17 mixed standards through screening, and the solvent of 17 mixed standards is 70% methanol water, and 17 standard samples of quality control products of the present invention and their use final concentrations are shown in Table 1:

[0026] Table 1

[0027]

[0028]

[0029]

[0030] The present invention is suitable for detecting human urine, serum, tissue, cells and other samples. Taking the detection of human serum as an example, the liquid phase mass spectrometry conditions for 17 mixed standard detection are: the solvent of 17 mixed standard substances is: 70% methanol aqueous solution ; Chromatographic column: C18 filler, column brand Wates ACQUITY HSS T3, i.d.2.1×100mm, 1.8um; mobile p...

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Abstract

The invention discloses a quality control material for metabolomics detection and a quality control method of the quality control material. The quality control material comprises mixed standard samples of 4,4'-methylene bis(2-chloroaniline), p-anisidine, L-tyrosine methyl ester, 3-chloroaniline, 2,4-dimethyl quinoline, sulfapyridine, atrazine, sulfadoxine, DL-leucine, N-benzoyl-L-tyrosine ethyl ester, 6-phenyl-2-thiouracil, N-(o-toluoyl) glycine, 2-methyl-5-nitroimidazole-1-ethanol, glycyrrhetinic acid, flavanone, Epsilon-caprolactone and 2-aminopyridine. 17 standard samples in the method comefrom different material categories and are very stable, simple in preparation and small in amount; and the quality control material disclosed by the invention can accurately reflect the state of a chromatograph or a mass spectrometer.

Description

technical field [0001] The invention belongs to the technical field of mass sample detection of metabolomics, and in particular relates to a quality control product for metabolomics detection and a quality control method thereof. Background technique [0002] Metabolomics is a discipline that studies the dynamic changes of biological endogenous small molecule metabolites before and after organisms are stimulated or disturbed by using chemical analysis techniques and quantitative methods, and determines the correlation between these changes and biological processes. [0003] Liquid chromatography-mass spectrometry is widely used in metabolomics detection due to its advantages of high throughput, high resolution, high sensitivity, suitable for detection of high boiling point, thermal instability and high molecular weight compounds. However, affected by factors such as the sample pre-processing method, the state of the instrument itself, and the environment, the instrument fluc...

Claims

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Application Information

Patent Timeline
27 Dec 2019
Publication
CN110618215A
IPC
G01N30/02; G01N30/72
CPC
G01N30/02; G01N30/7233; G01N2030/027
Inventors
唐堂; 胡梦婷