Manufacture method and use method of rat tracheal intubation equipment

Abstract

The invention belongs to manufacture method and use method of rat tracheal intubation equipment, and particularly relates to a manufacture method and use method of tracheal intubation equipment of a rat acute myocardial infarction model. A needle core and an inner guide wire of the needle core are respectively a guide needle and a loach guide wire (Terumo super-slippery M-shaped loach guide wire in Japan) used for coronary artery intervention; the soft end of the guide wire penetrates through the tail end of the guide needle and is exposed from the head end of the guide needle by 0.4cm; and the redundant guide wire at the tail end of the guide needle is cut off. An outer-layer hose is prepared from an infusion needle of disposable venous infusion apparatus (Shanghai Youyu Medical EquipmentCo., Ltd., 0.5x20 infusion apparatus), wherein the length of the outer-layer hose is shorter than the length of a catheter needle by 0.3 cm, and the root of the hose is cut obliquely by a movable flap which accounting for 1 / 3 of the circumference of the hose. After anaesthesia, a rat is fixed and is subjected to skin preparation, a rat tongue is clamped out with a small tweezer, the tongue tip ispulled by a left hand, and a tracheal intubation pipe is hold by a right hand. A hard catheter needle at the head end of the tracheal intubation needle core is arranged at the root part of the rat tongue, and a trachea opening of the rat is searched by fine adjustment of the self-made tracheal intubation pipe under direct vision. The guide wire at the head end of the tracheal intubation pipe is sent in instantly when the trachea opening is opened, then the guide wire at the head end is inserted into the trachea opening by about 1cm through rotation, and the catheter needle is pulled out, wherein if the rat can breathe uniformly and smoothly, intubation is successful.

Description

technical field [0001] The invention belongs to the manufacture and use of a rat tracheal intubation device, in particular to the manufacture and use of a rat acute myocardial infarction model production tracheal intubation device. Background technique [0002] Endotracheal intubation is an essential device in the determination of respiratory function, respiratory drug administration and drug efficacy identification, modeling of respiratory diseases, and other experiments that require ventilator support. Studies have shown that tracheal intubation has always been one of the main factors affecting the death of acute myocardial infarction models in rats. Traditional tracheal intubation through neck incision is more harmful and complicated to operate. Oral intubation under direct vision has less damage and is easy to operate, and is widely used. However, oral intubation with the traditional No. 7 mouse gavage needle is not easy to succeed. Some scholars have shown that the mort...

AI Technical Summary

Problems solved by technology

Studies have shown that tracheal intubation has always been one of the main factors affecting the death of rat models of acute myocardial infarction. Traditional intubation through neck incision is more harmful and complicated to operate.

Oral intubation under direct vision has less damage and is easy to operate, and is widely used. However, oral intubation with the traditional No. 7 mouse gavage needle is not easy to succeed. Some scholars have shown that the mortality rate of oral intubation is higher than that of Endotracheal intubation through neck incision may be related to unskilled operation and selection of endotracheal intubation equipment

The mouse gavage needle is hard and the head end is rough. In the previous experiment, I found that the following shortcomings were exposed: the rat's throat and trachea were damaged during intubation; moving the rat during the operation increased the damage to the trachea; Poor contact, the tidal volume of the ventilator is difficult to control

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