A preparation method of anticoagulant fibrinolytic nanofiber material

A nanofiber, anticoagulant technology, applied in the field of biomedical engineering, can solve problems such as limiting the application of heparin, and achieve the effect of improving the binding rate, anti-inflammatory, antipyretic and analgesic, and preventing thrombosis.

Active Publication Date: 2021-10-12
广州大鱼创福科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the anticoagulant activity of heparin, a large amount of heparin will cause side effects such as bleeding and induction of thrombocytopenia, which greatly limits the clinical application of heparin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] The invention provides a preparation method of anticoagulant fibrinolytic nanofiber material, comprising the following steps:

[0026] S1: Dissolve chitosan, polyethylene oxide and acetylsalicylic acid in an acetic acid solution, and stir to obtain a core spinning solution;

[0027] S2: Dissolve chitosan, polyethylene oxide and lumbrokinase in acetic acid solution, and stir to obtain chitin spinning solution;

[0028] S3: using the core spinning solution as the core and the chitin spinning solution as the shell to perform coaxial electrospinning to obtain acetylsalicylic acid / lumbrokinase nanofibers;

[0029] S4: washing the acetylsalicylic acid / lumbrokinase nanofibers with DMEM medium to a pH of 7.2, and then lyophilized and activated by a plasma processor to obtain activated nanofibers;

[0030] S5: soaking the activated nanofibers in DMEM medium containing low molecular weight heparin sodium, performing negative pressure flash explosion, performing grafting reaction...

Embodiment 1

[0044] S1. The viscosity average molecular weight of 0.5g is 5.0×10 5 , Chitosan with 80% degree of deacetylation, 1.5g average molecular weight 1.0×10 6 The polyethylene oxide and 0.8g of acetylsalicylic acid were completely dissolved in 100ml of 90% (v / v) acetic acid solution, and after stirring evenly, the core spinning solution was obtained;

[0045] S2, the viscosity average molecular weight of 0.5g is 5.0×10 5 , Chitosan with 80% degree of deacetylation, 1.5g average molecular weight 1.0×10 6 The polyethylene oxide and 0.05g of lumbrokinase with an enzyme activity of 8U / mg were completely dissolved in 100ml of 90% (v / v) acetic acid solution, and after stirring evenly, the chitin spinning solution was obtained;

[0046] S3. Use the core spinning solution as the core and the chitin spinning solution as the shell to perform coaxial electrospinning, and the needle specification is a coaxial needle; the electrospinning conditions are, the voltage is 15KV, the distance is 12...

Embodiment 2

[0050] S1. The viscosity average molecular weight of 0.5g is 5.0×10 5 , Chitosan with 80% degree of deacetylation, 0.5g average molecular weight 1.0×10 6 The polyethylene oxide and 0.5g of acetylsalicylic acid were completely dissolved in 100ml of 90% (v / v) acetic acid solution, and after stirring evenly, the core spinning solution was obtained;

[0051] S2, the viscosity average molecular weight of 0.5g is 5.0×10 5 , Chitosan with 80% degree of deacetylation, 0.5g average molecular weight 1.0×10 6 The polyethylene oxide and 0.01g of lumbrokinase with an enzyme activity of 5U / mg were completely dissolved in 100ml of 90% (v / v) acetic acid solution, and after stirring evenly, the chitin spinning solution was obtained;

[0052] S3. Use the core spinning solution as the core and the chitin spinning solution as the shell to perform coaxial electrospinning, and the needle specification is a coaxial needle; the electrospinning conditions are, the voltage is 12KV, the distance is 7c...

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Abstract

The invention discloses a preparation method of anticoagulant fibrinolytic nanofiber material, which comprises (1) completely dissolving chitosan, polyethylene oxide and acetylsalicylic acid in 90% acetic acid solution, and stirring evenly to obtain core fiber silk liquid; (2) fully dissolving chitosan, polyoxyethylene and lumbrokinase in 90% acetic acid solution, stirring evenly to obtain chitin spinning solution; (3) adopting coaxial electrospinning to obtain acetylsalicylic acid Acid / lumbrokinase nanofibers; (3) acetylsalicylic acid / lumbrokinase nanofibers were washed to neutral with DMEM medium, and then activated by plasma processor after lyophilization; (4) activated nanofibers were soaked in low Negative-pressure flash explosion in DMEM medium of sodium heparin, followed by grafting reaction, centrifugation and freeze-drying. The anticoagulant fibrinolytic nanofiber provided by the invention can better prevent thrombus formation.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a preparation method of an anticoagulant fibrinolytic nanofiber material. Background technique [0002] Anticoagulation and fibrinolysis are the main treatment methods for cardiovascular and cerebrovascular embolism, and heparin is a commonly used anticoagulant in the treatment of cardiovascular and cerebrovascular embolism. Heparin is a mucopolysaccharide formed by alternating 1→4 glycosidic linkages of hexuronic acid (L-iduronic acid, D-glucuronic acid) and D-glucosamine sulfate, linear with repeating units of hexasaccharides or octasaccharides. Chain structure, its molecular weight is between 3000-37000Da, used as anticoagulant and antithrombotic agent in medicine. In addition, heparin also has various biological functions such as anti-inflammatory, anti-allergic, anti-viral, anti-cancer, and blood lipid regulation. However, due to the anticoagulant activity of heparin,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01F8/18D01F8/16D01F1/10A61L33/10A61L33/04A61L33/16
CPCA61L33/0011A61L33/0047A61L33/04A61L33/08A61L2300/216A61L2300/232A61L2300/254A61L2300/42D01F1/10D01F8/16D01F8/18C08L5/10
Inventor 汤佳鹏葛彦刘希文朱俐
Owner 广州大鱼创福科技有限公司
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