A preparation method of anticoagulant fibrinolytic nanofiber material
A nanofiber, anticoagulant technology, applied in the field of biomedical engineering, can solve problems such as limiting the application of heparin, and achieve the effect of improving the binding rate, anti-inflammatory, antipyretic and analgesic, and preventing thrombosis.
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[0025] The invention provides a preparation method of anticoagulant fibrinolytic nanofiber material, comprising the following steps:
[0026] S1: Dissolve chitosan, polyethylene oxide and acetylsalicylic acid in an acetic acid solution, and stir to obtain a core spinning solution;
[0027] S2: Dissolve chitosan, polyethylene oxide and lumbrokinase in acetic acid solution, and stir to obtain chitin spinning solution;
[0028] S3: using the core spinning solution as the core and the chitin spinning solution as the shell to perform coaxial electrospinning to obtain acetylsalicylic acid / lumbrokinase nanofibers;
[0029] S4: washing the acetylsalicylic acid / lumbrokinase nanofibers with DMEM medium to a pH of 7.2, and then lyophilized and activated by a plasma processor to obtain activated nanofibers;
[0030] S5: soaking the activated nanofibers in DMEM medium containing low molecular weight heparin sodium, performing negative pressure flash explosion, performing grafting reaction...
Embodiment 1
[0044] S1. The viscosity average molecular weight of 0.5g is 5.0×10 5 , Chitosan with 80% degree of deacetylation, 1.5g average molecular weight 1.0×10 6 The polyethylene oxide and 0.8g of acetylsalicylic acid were completely dissolved in 100ml of 90% (v / v) acetic acid solution, and after stirring evenly, the core spinning solution was obtained;
[0045] S2, the viscosity average molecular weight of 0.5g is 5.0×10 5 , Chitosan with 80% degree of deacetylation, 1.5g average molecular weight 1.0×10 6 The polyethylene oxide and 0.05g of lumbrokinase with an enzyme activity of 8U / mg were completely dissolved in 100ml of 90% (v / v) acetic acid solution, and after stirring evenly, the chitin spinning solution was obtained;
[0046] S3. Use the core spinning solution as the core and the chitin spinning solution as the shell to perform coaxial electrospinning, and the needle specification is a coaxial needle; the electrospinning conditions are, the voltage is 15KV, the distance is 12...
Embodiment 2
[0050] S1. The viscosity average molecular weight of 0.5g is 5.0×10 5 , Chitosan with 80% degree of deacetylation, 0.5g average molecular weight 1.0×10 6 The polyethylene oxide and 0.5g of acetylsalicylic acid were completely dissolved in 100ml of 90% (v / v) acetic acid solution, and after stirring evenly, the core spinning solution was obtained;
[0051] S2, the viscosity average molecular weight of 0.5g is 5.0×10 5 , Chitosan with 80% degree of deacetylation, 0.5g average molecular weight 1.0×10 6 The polyethylene oxide and 0.01g of lumbrokinase with an enzyme activity of 5U / mg were completely dissolved in 100ml of 90% (v / v) acetic acid solution, and after stirring evenly, the chitin spinning solution was obtained;
[0052] S3. Use the core spinning solution as the core and the chitin spinning solution as the shell to perform coaxial electrospinning, and the needle specification is a coaxial needle; the electrospinning conditions are, the voltage is 12KV, the distance is 7c...
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