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Use of ENO2 autoantibody detection reagent in preparation of lung cancer screening kit

A technology of autoantibodies and kits, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of no ENO2 protein, no lung cancer, etc., and achieve good application prospects and effective screening effects

Active Publication Date: 2019-12-31
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no related reports on ENO2 protein autoantibodies, and there is no prior art related to lung cancer

Method used

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  • Use of ENO2 autoantibody detection reagent in preparation of lung cancer screening kit
  • Use of ENO2 autoantibody detection reagent in preparation of lung cancer screening kit
  • Use of ENO2 autoantibody detection reagent in preparation of lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Relationship between ENO2 autoantibodies in plasma and lung cancer

[0027] 1. Clinical data

[0028] 59 cases of lung cancer patients and 29 cases of healthy controls were selected. The basic information is as follows:

[0029] Basic Information lung cancer patients healthy control number of people 59 29 age 54.5±7.6 42±8.9 male ratio 37(62.7％) 13(46.7％)

[0030] 2. Detection principle

[0031] HuProt TM ENO2 protein is immobilized on the human protein custom chip (the ENO2 protein used is a full-length protein, and the protein sequence number is ENSP00000229277). After incubation with serum, ENO2 autoantibodies (mainly including IgG and IgM antibodies, and some other types Antibodies) will bind to it, wash to remove unbound antibodies and other proteins, and then detect with anti-human IgM fluorescently labeled secondary antibody (cy5-labeled, red) and anti-human IgG fluorescent secondary antibody (cy3-labeled, green...

Embodiment 2

[0048] Embodiment 2 The composition of detection kit of the present invention and its using method

[0049] 1. Kit composition

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Abstract

The invention relates to the field of in vitro diagnostic reagents, in particular to a use of an ENO2 autoantibody detection reagent in the preparation of a lung cancer screening kit. It is discoveredfor the first time in the use provided by the invention that, the autoantibody level of ENO2 protein in the serum of a lung cancer patient is significantly lower than that of a healthy patient. According to the use provided by the invention, the reagent for detecting the ENO2 protein autoantibody is applied to the preparation of the lung cancer screening kit, and then effective screening of lungcancer can be realized.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of ENO2 autoantibody detection reagents in the preparation of lung cancer screening kits. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung cancer ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68G01N33/535
CPCG01N33/535G01N33/57423G01N33/57488G01N33/6854G01N33/6884G01N2333/4746
Inventor 张立李为民
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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