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Areca nut necrotic ring spot virus and detection method thereof

A technology of ring spot virus and detection method, which is applied in the field of microbiology, can solve the problems of no nucleic acid and detection method, and achieve the effect of great value

Active Publication Date: 2020-01-03
HAINAN UNIVERSITY
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Problems solved by technology

At present, there are no reports of this genus of viruses at home and abroad, and there is no nucleic acid detection method for this genus of viruses

Method used

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  • Areca nut necrotic ring spot virus and detection method thereof
  • Areca nut necrotic ring spot virus and detection method thereof
  • Areca nut necrotic ring spot virus and detection method thereof

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Embodiment Construction

[0026] The present invention will be further described below in combination with specific embodiments, so as to better understand the present invention.

[0027] The virus of the present invention is isolated from betel nut leaves, and the virus is named as arecapalm necrotic ring-spot virus (Arecapalm necrotic ring-spot virus) ANRSV-XC1, and was preserved in the China Center for Type Culture Collection on June 17, 2018, with a preservation number For CCTCC NO: V201829. The full genome sequence of the virus has been uploaded to the National Center for Biotechnology Information, numbered MH395371.

[0028] Potatoviridae is the second largest virus family after Geminiviridae. Most viruses in this family have a wide range of hosts and often bring serious harm to agricultural production. According to the classification system revised by the International Committee Taxonomy of Viruses in 2017, the family includes 8 genera. The virus of the present invention is cloned and sequence...

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Abstract

The invention belongs to the technical field of biology, provides an areca nut necrotic ring spot virus, and further provides a detection method of the areca nut necrotic ring spot virus. The virus isnamed areca nut necrotic ring spot virus ANRSV-XC1, and the preservation number is CCTCC NO: V201829. The detection method includes three steps of establishing primer sequences required for nucleic acid detection, establishing detection reaction types and establishing a detection reaction system and reaction conditions. The virus isolated from leaves of areca nuts is significantly different fromreported viruses of a potato Y virus family, is a new virus genus of the potato Y virus family, provides a research basis for the research of an areca nut necrotic ring spot, and has great use value in scientific research and cash crop protection. In addition, according to the detection method, the areca nut necrotic ring spot virus can be accurately and quickly detected, and the detection methodis efficient and reliable and has great value in scientific research and cash crop protection.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to betel nut necrotic ringspot virus and a detection method thereof. Background technique [0002] Betel nut is the second largest tropical economic crop next to rubber in Hainan. It is called "green gold". The planting area is nearly 1.4 million mu, and its output accounts for 95% of the country. This is a living thing, and its pest control has always been one of the most concerned issues of the government, farmers and scientific research institutions, and plant virus diseases are currently the most harmful and difficult to control among diseases. In a recent survey, it was found that in Ding'an, Wanning, Baoting and other places, the incidence of betel nut necrotic ring spot virus disease was over 30%. The virus isolated from betel nut leaves was cloned and sequenced and entered the National Center for Biotechnology Information In comparison, it was found that it was signi...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12Q1/70C12Q1/686C12N15/11
CPCC12N7/00C12Q1/701C12Q1/686C12N2770/14021Y02A50/30
Inventor 崔红光杨柯
Owner HAINAN UNIVERSITY
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