Kit for assaying echinococcus in lesion tissue or dog feces by multiplex recombinase-aid amplification (RAA) and multiple PCR as well as assay method

A technology of Echinococcus multilocularis and Echinococcus, which is applied in the fields of agriculture and animal quarantine, can solve the problems of high cost, time-consuming and laborious, etc., and achieve the effects of saving detection costs, avoiding high false positives, and improving amplification performance
CN110656187AActive Publication Date: 2020-01-07中国疾病预防控制中心寄生虫病预防控制所国家热带病研究中心
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
中国疾病预防控制中心寄生虫病预防控制所国家热带病研究中心
Publication Date
2020-01-07

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Abstract

The invention discloses a kit for assaying echinococcus in lesion tissue or dog feces by multiplex recombinase-aid amplification (RAA) and multiple PCR. Meanwhile, the kit is also used for assaying echinococcus granulosus G1 and echinococcus multilocularis. The kit comprises primers as follows: echinococcus granulosus specific primers shown in SEQ ID NO.1 and SEQ ID NO.2 and echinococcus multilocularis specific primers shown in SEQ ID NO.3 and SEQ ID NO.4. In addition, the invention further discloses a multiplex RAA and multiplex PCR assay method for the echinococcus granulosus G1 and echinococcus multilocularis. Various verifications prove that the kit has characteristics of rapidness, sensitivity and high specificity, complex steps for repeated assay are eliminated, two genes can be assayed simultaneously in one reaction, functions of pathogens are made clear, the workload is reduced greatly, the assay time is shortened, the assay cost is reduced, and the kit has a greater development trend.
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Description

technical field

[0001] The invention relates to the technical fields of agriculture and animal quarantine, in particular to a detection method for Echinococcus granulosus G1 and Echinococcus multilocularis, and in particular to a multiple recombination enzyme for Echinococcus granulosus G1 and Echinococcus multilocularis Mediated amplification technology (Recombinase-aid Amplification, RAA) and multiplex PCR detection methods and kits. Furthermore, the present invention relates to primers for the simultaneous detection of Echinococcus granulosus G1 and Echinococcus multilocularis. Background technique

[0002] Echinococciosis, also known as hydatidosis, is a chronic parasitic disease caused by the larvae of Echinococcus tapeworm parasitizing human tissues and organs, and it is also an important zoonotic disease. Echinococcus has a complex life cycle, a wide range of epidemics and many hosts, and the intermediate hosts and final hosts are also different in different countrie...

Claims

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