Coated solid phase fragment and preparation thereof
A fragment and coating technology, applied in the field of coated solid phase fragments and their preparation
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Examples
Embodiment 1
[0089] Example 1: Process for the preparation of biomaterial-coated solid phase fragments by means of a pre-separation step
[0090] Dimensions will be 76 x 26 x 0.15mm 3 The solid-phase support of ® was equipped on a membrane (Ultron Systems dicing tape 1009R) and pulverized. Hold the lifting head of choice, such as the head of a cylindrical head screw (4.4mm diameter), in the vise as vertically as possible. Manually guide the containing fragmented solid phase support (with format 4.8×4.8×0.15mm) on the lifting head 3 ) to center the lifting head below the individual fragments. Manually place the membrane slowly and as horizontally as possible on the lifting head ( Figure 4 A). Physical pressure is exerted on the targeted debris by evenly squeezing the membrane onto the lifter head. The pressure was increased until the fragments separated from the adjacent fragments of the membrane ( Figure 4 B and C). Separation is made apparent by brightening of the separated membr...
Embodiment 2
[0092] Example 2: Tests with various lifting head variants
[0093] In another series of experiments, the use of various lifting head variants in the above method according to the invention was investigated. Ballpoint pen refills, cylindrical head screws, 3D printer heads, alignment pins and pipette tips were investigated as lifting head variants ( image 3 ).
[0094] Found in square format 5.0 x 5.0 x 0.15mm 3 In the case, the debris is evenly lifted from the membrane beyond a certain diameter of the lift head. Below this size of the lifting head, the debris is separated from the membrane obliquely and has to be removed from the membrane also obliquely (eg by means of suction cups).
[0095] The lifting head used has a diameter of 1.0-1.5mm capable of 2.5 x 2.5 x 0.15mm 3 -5.0×20.0×0.15mm 3 Singularize fragments on all test formats.
[0096] In order to reduce the friction of the lifting head on the membrane, especially in the case of elongated fragments, it has been f...
Embodiment 3
[0097] Example 3: Preparation of larger solid phase fragments using the method according to the invention
[0098] The maximum preparable solid phase fragment sizes using the method according to the invention were compared with those prepared using the method according to EP 1718948 B1 ( Image 6 ). In both cases membrane Ultron Systems 1009R and a 0.15 mm thick solid support were used.
[0099] Using the method according to EP 1718948 B1, 3.2 x 3.2mm can be produced in a reproducible manner 2 side length and therefore 10.24mm 2 Largest fragment of surface area. Using the method according to the invention, it is possible to prepare 2 Side length and 125mm 2 surface area of fragments. This corresponds to more than 12.2 times the surface area of the largest fragment prepared according to the method of EP 1718948 B1. No studies have been conducted to prepare even larger fragments by means of the method according to the invention.
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Abstract
Description
Claims
Application Information
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