New drug for preventing and controlling Spodoptera litura, and use method thereof

A technology for Spodoptera litura and new medicines, applied in the directions of botanical equipment and methods, applications, pesticides, etc., can solve the problem of lack of new pesticides and active molecules for controlling Spodoptera litura, and achieve the effect of good insecticidal activity

Active Publication Date: 2020-01-17
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the current lack of new pesticides and active molecules for preventing and controlling Spodoptera litura, the present invention finds that cyclosporine A has good insecticidal and inhibitory activity against Spodoptera litura

Method used

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  • New drug for preventing and controlling Spodoptera litura, and use method thereof
  • New drug for preventing and controlling Spodoptera litura, and use method thereof
  • New drug for preventing and controlling Spodoptera litura, and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: The dosage and control method of cyclosporin A to control the second instar larvae of Spodoptera litura

[0021] 1.1 Tested Spodoptera litura

[0022] The second instar larva of Spodoptera litura.

[0023] 1.2 Cyclosporin A concentration

[0024] Cyclosporine A dosage: 50 μM, 100 μM and 150 μM.

[0025] 1.3 Bioassay methods

[0026] This application adopts the method of medicament mixed artificial feed (the feed composition mainly includes corn meal, soybean meal, yeast meal, sucrose, vitamins, etc., and the formula refers to formula 4 in Liang Gemei et al. (1999)) for bioassay. That is, the artificial feed of Spodoptera litura is first configured, and before the feed is cooled and solidified, different doses of cyclosporin A are mixed into the artificial feed of Spodoptera litura, then the feed is cut into pieces and placed in a 750 mL plastic box for experiment. Put 16 2nd instar larvae in a small plastic box. Set up three repetitions, check the death of larvae on ...

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PUM

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Abstract

The invention belongs to the field of toxicological activity of pest prevention and control, and particularly relates to a new drug for preventing and controlling Spodoptera litura, and a use method thereof, wherein the new drug is cyclosporin A, and the Spodoptera litura refers to second-instar and third-instar Spodoptera litura larvae. According to the invention, with the application of the newdrug in prevention and control of Spodoptera litura, the lethal concentration of the cyclosporin eaten by Spodoptera litura larvae is 100-150 [mu]M; the test results show that the biological source agent has good insecticidal activity on agricultural pests Spodoptera litura; cyclosporin A acts on Spodoptera litura, so that the activity of dephosphorylase is possibly inhibited so as to affect the expression and the activity of the antibacterial peptide and the detoxification enzyme in insects; and cyclosporin A or the compound with the similar chemical structure can possibly be the novel insecticide or can be used as a novel insecticidal functional group to be applied to development and utilization of novel pesticides.

Description

Technical field [0001] The invention belongs to the field of toxicological activity for pest control, and particularly refers to a new medicine for controlling Spodoptera litura and its use method. Background technique [0002] Prodenia litura [scientific name: Spodoptera litura (Fabricius)] It is a species of the genus Spodoptera of the Lepidoptera Noctuidae. The larvae feed on nearly 300 species such as sweet potato, cotton, taro, lotus, sesbania, soybean, tobacco, sugar beet and cruciferous and solanaceous vegetables. The leaves of plants are rampant intermittently. The larvae have a total of 6 instars, and enter the binge eating period after the 4th instar. When they are rampant, they can eat up a large area of ​​host plant leaves and migrate to other places for damage. Spodoptera litura occurs throughout the country and is a gluttony pest, mainly in Jiangxi, Jiangsu, Hunan, Hubei, Zhejiang, and Anhui in the Yangtze River basin; Henan, Hebei, and Shandong in the Yellow Rive...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/713A01N37/46A01P7/04
CPCA01N37/46A01N43/713
Inventor 刘晓光关若冰杨朔安世恒魏纪珍
Owner HENAN AGRICULTURAL UNIVERSITY
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