New drug for preventing and controlling Spodoptera litura, and use method thereof
A technology for Spodoptera litura and new medicines, applied in the directions of botanical equipment and methods, applications, pesticides, etc., can solve the problem of lack of new pesticides and active molecules for controlling Spodoptera litura, and achieve the effect of good insecticidal activity
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[0020] Example 1: The dosage and control method of cyclosporin A to control the second instar larvae of Spodoptera litura
[0021] 1.1 Tested Spodoptera litura
[0022] The second instar larva of Spodoptera litura.
[0023] 1.2 Cyclosporin A concentration
[0024] Cyclosporine A dosage: 50 μM, 100 μM and 150 μM.
[0025] 1.3 Bioassay methods
[0026] This application adopts the method of medicament mixed artificial feed (the feed composition mainly includes corn meal, soybean meal, yeast meal, sucrose, vitamins, etc., and the formula refers to formula 4 in Liang Gemei et al. (1999)) for bioassay. That is, the artificial feed of Spodoptera litura is first configured, and before the feed is cooled and solidified, different doses of cyclosporin A are mixed into the artificial feed of Spodoptera litura, then the feed is cut into pieces and placed in a 750 mL plastic box for experiment. Put 16 2nd instar larvae in a small plastic box. Set up three repetitions, check the death of larvae on ...
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