Praxelis clematidea phenylpropanoid compounds and application thereof

A technology of phytophenanthine and a compound, applied in the field of medicine, can solve problems such as insufficient research on chemical constituents of Pseudomonas spp., and achieve the effect of good in vitro anti-inflammatory activity

Inactive Publication Date: 2020-01-17
GUANGDONG PHARMA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the existing research on the chemical constituents of Pseudomonas is not thorough enough, the specific structure and pharmaceutical functions of its chemical constituents are worthy of further research and development, so as to provide a new way for the resource utilization of this exotic vicious weed

Method used

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  • Praxelis clematidea phenylpropanoid compounds and application thereof
  • Praxelis clematidea phenylpropanoid compounds and application thereof
  • Praxelis clematidea phenylpropanoid compounds and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] In this Example 1, two compounds were obtained by extracting and separating from Pseudomonas spp., which were identified, and the specific structures of the two compounds were confirmed. The extraction and separation method included the following steps:

[0028] (1) Cut 10kg of the whole plant of Stinkia officinalis naturally dried in the shade, and use 75% ethanol for reflux extraction at 60° C. for 4 times, each time for 2 hours, to obtain an extraction concentrate (10L).

[0029] (2) The extraction concentrate was successively extracted three times with equal volumes of cyclohexane, chloroform, ethyl acetate and n-butanol to obtain a chloroform layer (74.68 g).

[0030] (3) The chloroform fraction (74.68 g) was subjected to silica gel column chromatography, followed by gradient elution with dichloromethane-methanol (100:0, 50:1, 20:1, 10:1, 5:1, 3:1 , 0:100; v / v), take the dichloromethane-methanol eluent with a volume ratio of 50:1 as the effective part X and Y;

[...

Embodiment 2

[0055] In this example, LPS (lipopolysaccharide) was used to induce BV-2 (mouse microglia) cells to establish an in vitro inflammation model. The cytotoxicity of the samples was tested by MTT, the production of NO was determined by Griess, the effects of the above compounds 1 and 2 on the release of inflammatory mediator NO from BV-2 cells induced by lipopolysaccharide were investigated, and the anti-inflammatory drug minocycline was used as the positive control.

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Abstract

The invention belongs to the technical field of medicine, and provides novel phenylpropanoid compounds extracted from praxelis clematidea. An animal pharmacodynamic test shows that the novel phenylpropanoid compounds exhibit an inhibitory effect on inflammatory medium NO produced by LPS-induced BV-2 cells, and have a significant anti-inflammatory effect and no toxicity to the cells, so that the praxelis clematidea phenylpropanoid compounds provided by the invention can be used to prepare drugs related to inflammation.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a pseudostinky phenylpropanoid compound and an application thereof. Background technique [0002] Inflammation is the defensive response of living tissue with vascular system to damaging factors. Most diseases are accompanied by the occurrence of inflammation. Inflammation will aggravate the occurrence and development of the disease. Some chronic inflammations may even lead to the occurrence of tumors. Therefore, the control and treatment of inflammation is of great significance. [0003] Natural medicines, especially those derived from plants, have chemical structure diversity and biological activity diversity, and have always been the main source of human disease prevention and treatment. Many clinically applied drugs are directly or indirectly derived from natural products. Natural products can not only be used as precursors for semi-synthesis of drugs, but also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D319/20C07C69/734A61P29/00A61P25/00A61P11/00A61P1/16A61P15/14A61P1/00A61P9/00A61P25/28
CPCA61P1/16A61P9/00A61P11/00A61P25/00A61P25/28A61P29/00C07B2200/07C07C69/736C07D319/20C07C2601/14
Inventor 何祥久王宜海肖露徐静雯
Owner GUANGDONG PHARMA UNIV
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