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Burkholderia arboris and application thereof

A Burkholderia and bacteria technology, applied in the field of bacterial culture and fermentation applications, to achieve the effects of increasing yield, improving nematode resistance, and improving commodity quality

Active Publication Date: 2020-01-17
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Anti-nematode activity of other Burkholderia species has not been reported

Method used

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  • Burkholderia arboris and application thereof
  • Burkholderia arboris and application thereof
  • Burkholderia arboris and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0036] The application of the bacterium of the present invention, the preparation method of the fermentation liquid of the bacterium comprises: preparation of beef extract peptone liquid medium → sterilization → cooling → inoculation → fermentation at 200-300 rpm on a shaking table for 12-72 hours → collection of bacterial liquid.

[0037] See Figure 5 , Figure 6 Shown; Further, the application method of the fermented liquid of this bacterium of the present invention comprises:

[0038] A. When cultivating crops, dig a cultivation hole in the soil corresponding to the size of the seedling roots, put the seedlings in, and pour the fermented bacterial liquid on the roots of the plants. The amount of bacterial liquid is 10ml-100ml / plant according to the size of the roots. ;or,

[0039] B. After mixing the fermented bacterial liquid with any proportion of root water, pour it into the cultivation hole when the crop is cultivated, or water it after covering it with soil; or,

...

Embodiment 1

[0053] Organize the tobacco field soil according to the routine requirements. Select robust, tidy tobacco seedlings with a plant height of about 10 cm, with a plant spacing of 50 cm and a nest depth of 15 cm. In each hole for tobacco seedling cultivation, 10ml of Burkholderia arboris J211 strain bacterial liquid was first applied, and then the tobacco was managed according to the tobacco seedling cultivation specifications by covering soil and watering, and the tobacco seedlings grew normally. After the tobacco plant grows, the effect of nematode control can be seen.

Embodiment 2

[0055] Select tomato seedlings that grow normally, and dig small holes beside the roots, deep to the side roots. In each hole, apply 50ml of Burkholderia arboris J211 strain bacterial liquid and then cover with soil, and then manage according to the normal management method of vegetable seedlings, it can be seen that the vegetable seedlings grow normally.

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Abstract

The invention relates to burkholderia arboris and application thereof. The bacterium is the burkholderia arboris, the Latin name is burkholderia arboris J211; the preservation number is CCTCC NO:M 2019747; the burkholderia arboris is separated from tobacco field soil in Kunming Yunnan; a culture medium of the burkholderia arboris is a beef extract peptone culture medium; and the burkholderia arboris is applied to preventing and treating of nemas. The burkholderia arboris has the beneficial effects that 1, the ability of significantly improving nemas resistance of field crops is achieved; 2, the commercial quality of the crops is improved; and 3, the yield of the crops is increased. The burkholderia arboris can further be used in combination with other nematode biocontrol bacterial agents so as to further enhance the preventing and treating effect of the burkholderia arboris agent.

Description

technical field [0001] The invention belongs to the technical field of methods for bacterial culture and fermentation application. Background technique [0002] Nematodes are a very common plant pest during the growth of crops. Some plant parasitic nematodes can damage the root tissue of plants. For example, root-knot nematodes can hinder the development of plant roots and nutrient absorption. Some nematodes can also spread other diseases. Nematodes are estimated to cost crops the equivalent of $100 billion a year. Root-knot nematodes (Meloidogyne spp.) and cyst nematodes (Heterodera spp.) are two types of nematodes that can seriously damage a variety of important crops. They are widely distributed in the world and cause serious damage. [0003] Common nematode control methods mainly include fumigation, the use of chemical pesticides, and the use of microbial agents that can kill nematodes. Fumigation has a huge destructive effect on the environment, and the country has e...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P5/00A01G7/06A01C21/00C12R1/01
CPCA01G7/06A01C21/00C12R2001/01C12N1/205
Inventor 陈穗云王兴红张仁军
Owner YUNNAN UNIV