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Human circulating tumor cell MSI (micro-satellite instability) detection primer set, kit and method

A detection kit and tumor cell technology, applied in the field of human circulating tumor cell MSI detection primer sets, to achieve the effect of strong specificity, high sensitivity, and optimized direct expansion system

Pending Publication Date: 2020-01-17
苏州绘真医学检验有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no domestic data pointing out that those sites Panel can more accurately indicate the status of MSI

Method used

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  • Human circulating tumor cell MSI (micro-satellite instability) detection primer set, kit and method
  • Human circulating tumor cell MSI (micro-satellite instability) detection primer set, kit and method
  • Human circulating tumor cell MSI (micro-satellite instability) detection primer set, kit and method

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Experimental program
Comparison scheme
Effect test

Embodiment

[0036] 1. Instruments and reagents

[0037] (1) Instrument

[0038] 1. PCR instrument

[0039] Bio-RAD C1000 96-well gradient amplification instrument (1851197).

[0040] 2, system

[0041] Currently, The system is the only product that has been approved by the FDA for marketing. The system uses the positive enrichment method of immunomagnetic beads coated with the tumor cell epithelial marker EpCAM antibody to isolate CTCs from circulating blood.

[0042] 3. ABI 3500 Dx Genetic Analyzer

[0043] (2) Reagents

[0044] 1. PBS 1L formula pH7.4:

[0045] Potassium dihydrogen phosphate (KH 2 PO 4 ): 0.24g,

[0046] Disodium hydrogen phosphate (Na 2 HPO 4 ): 1.44g,

[0047] Sodium chloride (NaCl): 8g,

[0048] Potassium chloride (KCl): 0.2g,

[0049] Add about 800mL of deionized water and stir well to dissolve, then add concentrated hydrochloric acid to adjust the pH to 7.4, and finally adjust the volume to 1L.

[0050]2. The present invention involves primers, ...

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Abstract

The invention discloses human circulating tumor cell MSI (micro-satellite instability) detection primer set, kit and method; the primer set can detect human MSI state with high sensitivity and specificity; the kit can rapidly, efficiently and accurately detect the MSI state of human circulating tumor cells; the method has the advantages that, for instance, the results are true and reliable, inter-chamber consistency is good, and the method is easy to popularize. According to the invention, circulating tumor cell detection is optimized, so that the detection results are high in sensitivity andaccuracy on the basis that the detection is noninvasive.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a primer set, a kit and a detection method for human circulating tumor cell MSI detection. Background technique [0002] Circulating tumor cells (CTCs) refer to all kinds of tumor cells that break away from the primary tumor site and metastatic site and enter the blood circulation. CTCs have obvious advantages in early detection of tumors, guidance of medication, detection of prognosis and recurrence, prompting of tumor metastasis, etc.: 1) CTCs are meaningful for early diagnosis of malignant tumors. A small amount of CTCs can be detected, but no CTCs can be detected in the blood of benign diseases and healthy subjects; 2) CTCs have similar genetic characteristics to tumor cells in the primary tumor, enabling non-invasive and sustainable monitoring of tumor progression , and guide medication; 3) Similar to the early detection of tumors, the prognosis can continuously monitor the re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6886C12Q1/6858C12Q2600/166C12Q2531/113C12Q2563/107C12Q2545/101C12Q2545/113C12Q2565/125
Inventor 张惠丹戴敬杨丽萍赵洪玉
Owner 苏州绘真医学检验有限公司
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