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A kit for detecting serum testosterone hormone by ultra-high performance liquid chromatography-tandem mass spectrometry

A technology of high performance liquid chromatography and tandem mass spectrometry, which is applied in the field of hormone detection to achieve the effect of low sample usage, improved precision and avoiding aggregation

Active Publication Date: 2020-06-16
天津汉科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing detection methods need to use a sample volume of not less than 500 μL to meet the signal response of the target in the serum sample

Method used

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  • A kit for detecting serum testosterone hormone by ultra-high performance liquid chromatography-tandem mass spectrometry
  • A kit for detecting serum testosterone hormone by ultra-high performance liquid chromatography-tandem mass spectrometry
  • A kit for detecting serum testosterone hormone by ultra-high performance liquid chromatography-tandem mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] An embodiment of the ultra-high performance liquid chromatography-tandem mass spectrometry of the present invention for detecting testosterone hormone in serum, adopting isotope dilution liquid chromatography-tandem mass spectrometry to detect testosterone hormone in serum samples includes the following parts:

[0045] 1. Materials

[0046] The sample involved in this example is fresh normal human serum, with a clear appearance and no hemolysis, jaundice and lipemia.

[0047] (1) Instruments: ultra-high performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS / MS, Waters, USA); vortex mixer (IKA, Germany); centrifuge (Thermo Fisher, USA) ; Nitrogen blowing instrument (Bona Aegel Company); electronic analytical balance (Germany Sartorius Instrument Systems Co., Ltd.).

[0048] (2) Reagent consumables: Methanol (chromatographically pure, Merck, Germany), acetonitrile (chromatographically pure, Merck, Germany), n-hexane (chromatographically pure, Tian...

Embodiment 2

[0072] 1. Materials

[0073] The samples involved in this example are all from outpatients and inpatients of a hospital in Tianjin. The samples are fresh human serum with a clear appearance and no hemolysis, jaundice and lipemia.

[0074] (1) Instruments: ultra-high performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS / MS, Waters, USA); vortex mixer (MS 3basic, IKA, Germany); centrifuge (eppendorf, Germany) ); Nitrogen Blowing Apparatus (Bona Aegel); One-thousandth Balance (Germany Sartorius Instrument Systems Co., Ltd.); Adjustable Pipette (Germany Eppendorf, 0.5~10μL, 10~100μL, 100~ 1000μL); glassware, beakers, graduated cylinders, etc.

[0075] (2) Reagent consumables: Methanol (chromatographically pure, Merck, Germany), acetonitrile (chromatographically pure, Merck, Germany), n-hexane (chromatographically pure, Tianjin Concord Technology Co., Ltd.), ethyl acetate (chromatographically pure, Tianjin Kang Kede Technology Co., Ltd.); formic acid (mas...

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Abstract

The invention provides a kit for detecting serum testosterone by means of ultra-high performance liquid chromatography-tandem mass spectrometry. The kit belongs to the technical field of hormone detection, and comprises a standard solution, an internal standard solution, a diluent, extracts, a dissociation agent, a stabilizer, a quality control material and a chromatographic column, wherein the extracts comprise a first extract and a second extract; the first extract is a mixed solution of ethyl acetate and normal hexane; the second extract is normal hexane; the dissociation agent is an ammonium acetate aqueous solution with a concentration of 0.4-0.6 mol / L; and the stabilizer is a sodium carbonate aqueous solution with the concentration of 0.1-0.3 mol / L. A linear range, a detection limit,a recovery rate and the precision of the testosterone content detected by adopting the kit meet the requirements, the kit is high in sensitivity, high in specificity, precise and reliable, and the kit can be applied to quantitative detection of testosterone in clinical serum samples.

Description

technical field [0001] The invention belongs to the technical field of hormone detection, in particular to a kit for detecting serum testosterone hormone by high performance liquid chromatography-tandem mass spectrometry. Background technique [0002] Testosterone is a steroid hormone. As an endogenous hormone and anabolic hormone, it can enhance muscle strength, enhance immunity, and fight against osteoporosis. Measurement of serum testosterone hormone has important implications in adult and pediatric endocrinology and oncology. In men, testosterone analysis is used primarily to confirm the diagnosis of hypogonadism, but also to evaluate boys with delayed puberty or precocious puberty and to monitor the adequacy of testosterone therapy. In women, testosterone analysis can be used to evaluate for hyperandrogenism (eg, idiopathic hirsutism, congenital adrenal hyperplasia, polycystic ovary syndrome, and androgen-secreting ovarian or adrenal tumors), and for androgen deficienc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/045G01N2030/062
Inventor 王彬彬
Owner 天津汉科生物科技有限公司
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