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Strain of pseudomonas aeruginosa producing 5-hydroxymethyl-2 furancarboxylic acid and application of strain of pseudomonas aeruginosa

A technology of Pseudomonas aeruginosa and furanic acid, applied in the biological field, achieves good application prospects, simple operation, and mild conditions

Active Publication Date: 2020-01-24
NANJING POLYTECHNIC INSITUTE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, 5-hydroxymethylfurfural is a kind of microbial inhibitor, and the microorganism that can grow in 5-hydroxymethylfurfural is less, and for utilizing 5-hydroxymethylfurfural to prepare 5-hydroxymethyl-2 furanic acid research not yet reported

Method used

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  • Strain of pseudomonas aeruginosa producing 5-hydroxymethyl-2 furancarboxylic acid and application of strain of pseudomonas aeruginosa
  • Strain of pseudomonas aeruginosa producing 5-hydroxymethyl-2 furancarboxylic acid and application of strain of pseudomonas aeruginosa
  • Strain of pseudomonas aeruginosa producing 5-hydroxymethyl-2 furancarboxylic acid and application of strain of pseudomonas aeruginosa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] This example illustrates Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) Isolation and Characterization of PC-1

[0034] Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) PC-1.

[0035] (1) After the soil sample was dissolved in deionized water, the supernatant was applied to the screening solid medium for cultivation. The cultivation temperature was 35 °C and the cultivation time was 24-48 h. This method can screen high-concentration 5-hydroxymethylfurfural-resistant microorganisms.

[0036] (2) Pick the strains obtained in step (1) into the enrichment culture medium for enrichment culture at a temperature of 35 °C, and obtain seed liquid after 12-14 hours of culture.

[0037] (3) The seed solution obtained in step (2) was inoculated in a liquid medium containing 100 mM 5-hydroxymethylfurfural at an inoculation volume of 0.5 mg / mL for transformation detection. The conversion temperature was 35 °C, the conversion time was 7 hours, and the rotation speed was 150 ...

Embodiment 2

[0041] This example illustrates the effect of 5-hydroxymethylfurfural concentration on Pseudomonas aeruginosa Pseudomonas aeruginosa The effect of growth amount of PC-1 and conversion rate of 5-hydroxymethylfurfural.

[0042]Seed solution was obtained by the method of Example 1. Add different concentrations of 5-hydroxymethylfurfural (50mM, 75mM, 100mM, 150mM, 200mM) to 5 bottles of liquid medium with equal volumes, and take 1 bottle without adding 5-hydroxymethylfurfural as a control. After mixing, each bottle was inoculated with an inoculation amount of 5 mg / mL, and after culturing at 150 rpm and 35 °C for 12 h, the concentration of Pseudomonas aeruginosa PC-1 was measured with a spectrophotometer, and the results were as follows: figure 2 As shown, with the increase of 5-hydroxymethylfurfural concentration, the growth of bacteria was gradually inhibited. When the initial concentration of 5-hydroxymethylfurfural is not higher than 100 mM, the growth of the bacteria is l...

Embodiment 3

[0044] This example illustrates the impact of different pH on the yield of 5-hydroxymethyl-2 furanic acid and the conversion rate of 5-hydroxymethylfurfural

[0045] After obtaining the seed solution according to the method of Example 1, inoculate the liquid medium containing 100 mM 5-hydroxymethylfurfural at different pH (5.5, 6.0, 6.5, 7.0, 7.5, 8.0) with an inoculation amount of 10 mg / mL After culturing at 150 rpm at 35°C for 6 h, samples were taken for HPLC detection. The result is as image 3 It was shown that when the pH was 7.0, the conversion rate of 5-hydroxymethylfurfural reached 100%, and the yield of 5-hydroxymethyl-2-furancarboxylic acid was the highest, reaching 89.2%, which was the optimal reaction condition.

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Abstract

The present invention discloses a strain of pseudomonas aeruginosa producing 5-hydroxymethyl-2 furancarboxylic acid and an application of the strain of the pseudomonas aeruginosa, and belongs to the technical field of biology. The disclosed pseudomonas aeruginosa PC-1 has a preservation number of CCTCC NO:M2019537. The strain is used to transform 5-hydroxymethylfurfural into 5-hydroxymethyl-2 furancarboxylic acid and has advantages of short reaction time, mild conditions, no pollution to the environment, and high product selectivity. At the same time, the present invention also provides an application of the strain in preparing the 5-hydroxymethylfurfural, product yield is high, and the strain has a broad application prospect.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to a kind of Pseudomonas aeruginosa ( Pseudomonas aeruginosa ), and the application of the strain in catalyzing the production of 5-hydroxymethyl-2-furanic acid from 5-hydroxymethylfurfural. Background technique [0002] With the increasing scarcity of petroleum resources and the intensification of global warming, in recent years, people have gradually shifted their attention to the development and utilization of bio-based energy and platform compounds. 5-Hydroxymethylfurfural (5-hydroxymethylfurfural, HMF) is an important bio-based platform compound, which is listed in the "Top 10+4" platform compounds announced by the U.S. Department of Energy. It has relatively active chemical properties and can prepare a variety of derivatives substances (Green Chem, 2017, 19, 4544). 5-Hydroxymethylfurfural can be selectively reduced to prepare 5-hydroxymethyl-2 furoic acid (5-hydroxymet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/04C12R1/385
CPCC12P17/04C12N1/205C12R2001/385
Inventor 常思源李冰峰潘鑫姚自选潘艳张媛曹亮
Owner NANJING POLYTECHNIC INSITUTE
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