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Application of levofloxacin antibiotic in preparation of sensitization drug for anticancer drugs and anticancer drug comprising levofloxacin antibiotic

A technology of levofloxacin and anticancer drugs, applied in the field of drug research, can solve the problems of drug resistance of cancer cells, large drug side effects, poor selectivity, etc.

Inactive Publication Date: 2020-02-07
KUNMING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the cancer chemotherapy drugs currently used clinically have many problems such as large toxic and side effects, uncertain curative effect, poor selectivity, weak targeting, and drug resistance of cancer cells. very restrictive

Method used

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  • Application of levofloxacin antibiotic in preparation of sensitization drug for anticancer drugs and anticancer drug comprising levofloxacin antibiotic
  • Application of levofloxacin antibiotic in preparation of sensitization drug for anticancer drugs and anticancer drug comprising levofloxacin antibiotic
  • Application of levofloxacin antibiotic in preparation of sensitization drug for anticancer drugs and anticancer drug comprising levofloxacin antibiotic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Levofloxacin antibiotics have significant anticancer effects in vitro.

[0055] In vitro anticancer test method: levofloxacin antibiotics were dissolved in sterile saline, and the in vitro anticancer test was carried out at the final concentration of 25, 50, 100, 200, and 400 μg / ml. Human cancer cells in the logarithmic growth phase were collected, suspended in DMEM / F12 complete culture solution without antibiotics with a volume ratio of 10% fetal bovine serum (FBS), and inoculated on a 96-well plate, adding 100 μl of cell suspension to each well (except MCF-7 was inoculated with 1×10 per well 4 1 cells, the rest of the cancer cell lines were inoculated in each well with 6×10 3 cells). 37°C, 5% CO 2 After culturing overnight in the incubator, suck out the culture medium, and then add 200 µl of culture medium containing corresponding concentrations of levofloxacin antibiotics according to the design (the culture medium is DMEM / F12 complete culture medium wi...

Embodiment 2

[0059] Example 2: Levofloxacin antibiotics have selective in vitro anticancer effects on some human cancer cells

[0060] The experimental method is the same as the in vitro anti-cancer experiment in Example 1, using human normal vascular endothelial cells ECV-304 to compare the selective cytotoxicity. In vitro cell culture experiments found that under the same dose and test conditions, levofloxacin antibiotics had significantly greater cytotoxic effects on human leukemia cells K-562, colon cancer cells HCT-116 and gastric cancer cells SGC-7901 than on normal human vascular endothelium. Cytotoxicity of cell line ECV-304, levofloxacin antibiotics have selective anticancer effect on some human cancer cells. see results Figure 4 .

Embodiment 3

[0061] Embodiment 3: Levofloxacin antibiotics have obvious anticancer effect in vivo

[0062] Anti-transplant human nasopharyngeal carcinoma test method in nude mice: prepare human nasopharyngeal carcinoma cell samples by cell culture, adjust the cell concentration to 4×10 with sterile, FBS-free, and antibiotic-free DMEM / F12 culture medium 7 cells / ml to obtain a cell suspension. For balb / c nude mice (body weight 19-21g) that had been adaptively fed for 3 days, each mouse was subcutaneously inoculated with 0.1ml of cell suspension on the right back. Nude mice were bred in SPF laboratory. Measure the long diameter (a) and transverse diameter (b) of each tumor-bearing mouse tumor with a vernier caliper with automatic reading, using the formula: V=a×b 2 / 2 to calculate the tumor volume per mouse. On the 8th day after cell inoculation, most mice had subcutaneous tumors visible to the naked eye, and the average tumor volume reached 200-250mm 3 Left and right, the tumor-bearing m...

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Abstract

The invention relates to application of levofloxacin antibiotic in preparation of a sensitization drug for anticancer drugs and an anticancer drug comprising the levofloxacin antibiotic, and belongs to the technical field of drug research. The levofloxacin antibiotic has significant in-vitro and in-vivo anticancer effects on multiple cancers of human; the anticancer effects of the levofloxacin antibiotic have an obvious dosage-effect and time-effect relations; when combined with other anticancer drugs for application, the levofloxacin antibiotic can significantly enhance the anticancer effectof the anticancer drug on various cancers, and the levofloxacin antibiotic and the other anticancer drug have synergistic or addition combined anticancer effects. In an effective in-vivo anticancer dosage, levofloxacin cannot remarkably affect weight development of animals. Results show that the levofloxacin antibiotic can be used as a broad-spectrum anticancer drug, particularly as the sensitization drug of other anticancer drugs, can be combined with other anticancer drugs, or form a combined anticancer drug composition with other anticancer drugs or can be used as a healthcare product for adjuvant therapy of cancers.

Description

technical field [0001] The present invention belongs to the technical field of drug research, and in particular relates to the application of levofloxacin antibiotics in the preparation of broad-spectrum anticancer drugs, the application of sensitizing drugs for other clinical anticancer drugs, the application of combined anticancer drug compositions, and Auxiliary anti-cancer health products. Background technique [0002] Malignant tumor is a major disease that seriously threatens human health and life safety. It has become the main cause of death of human beings and seriously threatens human health and life safety. According to data released by the International Cancer Institute of the World Health Organization in Globocan, there were 12.661 million new cancer cases and 7.564 million deaths worldwide in 2008. In 2012, there were 14.1 million new cancer cases worldwide, 8.2 million deaths, and 32.6 million cancer survivors. In 2015, there were 17.5 million new cancer case...

Claims

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Application Information

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IPC IPC(8): A61K31/5383A61K45/06A61K33/243A61K31/513A61P35/00A61P35/02
CPCA61K31/5383A61K45/06A61K33/243A61K31/513A61P35/00A61P35/02A61K2300/00
Inventor 贺小琼
Owner KUNMING MEDICAL UNIVERSITY
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