Preparation method of hEGF-rich lyophilized powder for repairing wound skin

A technology of skin repair and freeze-dried powder, which is applied in the biological field, can solve the problems of donor sources and immune rejection restrictions, and achieve the effects of improving physiological activity and metabolism, reasonable process design, and delaying aging

Inactive Publication Date: 2020-02-07
伯仕利生物科技发展(盐城)有限公司
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  • Abstract
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Problems solved by technology

At present, the commonly used clinical drugs for the treatment of ordinary burns are mainly anti-inflammatory drugs and traditional Chinese medicine ointments, but for large-scale

Method used

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  • Preparation method of hEGF-rich lyophilized powder for repairing wound skin
  • Preparation method of hEGF-rich lyophilized powder for repairing wound skin

Examples

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Embodiment 1

[0053] S1: Preparation of mesenchymal stem cells:

[0054] S11: Under sterile conditions, take 20 cm of the umbilical cord near the fetal end, cut off 2 cm at each end, rinse the umbilical cord 4-5 times with normal saline containing 200 U of penicillin and streptomycin until there is no blood, and then rinse with normal saline without double antibiotics , put it in a petri dish containing a little DMEM / F12 medium, cut it into small pieces of 2-3cm, cut the small pieces longitudinally, remove the internal arteries and veins of the umbilical cord, tear the tissue pieces into thin strips with tissue forceps, put them into In a petri dish of a small amount of DMEM / F12 medium, use ophthalmic scissors to cut into 1-2mm 3 fragments, the whole process was carried out in an ice bath;

[0055] S12: Dip the shredded tissue pieces with a little serum, transfer them into air-permeable T25 culture bottles, 20 per bottle, add 1.5ml of DMEM / F12 medium containing 20% ​​fetal bovine serum, ch...

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Abstract

The invention discloses a preparation method of hEGF-rich lyophilized powder for repairing wound skin. The lyophilized powder is prepared by lyophilizing culturing supernatant liquid which contains anover-expression hEGF gene and is applied to production of stem cells; the cell concentration of the culturing supernatant liquid is more than or equal to 1x10<7>/ml; and the lyophilized powder contains the over-expression hEGF, human keratinocyte growth factors, human fibroblastic growth factors, human platelet-derived growth factors and fibronectin. The invention discloses the preparation methodof the hEGF-rich lyophilized powder for repairing the wound skin. The process is reasonable in design and is simple in operation; and the prepared lyophilized powder cannot only be applied to repairing treatment of wounds, including burns and scalds, long lasting healing of ulcer wound surfaces and the like, of patients; and the method is simple and feasible, is applied to large-scale productionof the culturing supernatant lyophilized powder for different sources of stem cells, and provides a new way for application of the stem cells to medical transformation career; and therefore, the method is higher in practicability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of hEGF-rich freeze-dried powder for wound skin repair. Background technique [0002] Skin wound healing refers to the healing process after the skin tissue is severed or damaged by external force, including the complex combination of skin tissue regeneration, granulation tissue hyperplasia and scar formation, showing the synergistic effect of various repair processes. Skin trauma includes skin abrasions, skin burns, and chronic ulcer wounds. Burns are mainly serious damages to the skin, mucous membranes, and deep skin tissues caused by heat, chemicals, electric energy, radiation, etc., among which thermal burns of the skin are more common. Relevant statistical results show that traffic accidents rank first in the annual death due to accidental injuries, followed by burns, and in traffic accidents, there are also many wounded who are more damaged because of comb...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K9/19A61P17/02C12N15/85C12N5/10C12N15/12C07K14/485
CPCA61K9/19A61K35/28A61P17/02C07K14/485C12N5/0665C12N15/85C12N2509/00C12N2510/02
Inventor 吴金芸徐文倩叶华衍张炬雷登
Owner 伯仕利生物科技发展(盐城)有限公司
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