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Cell cycle detection method, and reagent and kit use by method

A cell cycle and detection method technology, applied in the direction of biochemical equipment and methods, measurement devices, microbial determination/inspection, etc., can solve problems such as time-consuming, labor-intensive, and time-consuming

Inactive Publication Date: 2020-02-07
菲诺克生物科技(上海)有限公司
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Problems solved by technology

However, after the whole test is prepared from the cells, 75% ethanol is added, and the cells are fixed and punched at the same time. It takes at least 24 hours to be tested on the machine, which has caused a lot of time loss for pharmaceutical companies’ research and development and scientific research.
And in method 3, ethanol fixation, PI staining and RNA digestion are carried out in three steps, which is time-consuming and labor-intensive.

Method used

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  • Cell cycle detection method, and reagent and kit use by method
  • Cell cycle detection method, and reagent and kit use by method
  • Cell cycle detection method, and reagent and kit use by method

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Embodiment Construction

[0032] Reference will now be made in detail to embodiments of the invention, one or more examples of which are described below. Each example is provided by way of explanation, not limitation of the invention. In fact, it will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the scope or spirit of the invention. For example, features illustrated or described as part of one embodiment can be used on another embodiment to yield a still further embodiment.

[0033] Thus, it is intended that the present invention cover such modifications and variations as come within the scope of the appended claims and their equivalents. Other objects, features and aspects of the invention are disclosed in or are apparent from the following detailed description. It is to be understood by those of ordinary skill in the art that the present discussion is a description of exemplary embodiments only, and is ...

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Abstract

The invention relates to the technical field of biology, in particular to a cell cycle detection method and a reagent and a kit used by the cell cycle detection method. The method comprises the step that saponin with a concentration of 0.06-0.3% (g / ml) is used for making contact with cells to enhance the permeability of cell membranes, so that DNA binding dye and ribonuclease can enter the cells and exert activity. According to the method, the detection time can be remarkably shortened, detection can be completed within about 10 minutes at the soonest, the detection accuracy is good, and the efficiency is high.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cell cycle detection method and reagents and kits used therefor. Background technique [0002] Eukaryotic cell division proceeds through a highly regulated cell cycle comprising successive phases termed Gl, S, G2 and M. Cell cycle progression is strictly controlled by the activation or shutdown of many cell cycle regulation-related signaling pathways (especially cyclin-related pathways) at specified times and spaces, which exhibit high dynamics during the cell cycle. For example, at a specific cell cycle stage, some proteins are transferred from the nucleus to the cytoplasm or vice versa, and some proteins are rapidly degraded, etc. [0003] Accurate determination of cell cycle state is a key requirement for studying cellular processes that affect the cell cycle or depend on cell cycle position. These assays are particularly critical in drug screening applications. [0004] Curr...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/44C12Q1/02
CPCG01N33/5005
Inventor 李佐青刘津陈大为王胜旺邓少华康恺
Owner 菲诺克生物科技(上海)有限公司
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