Nucleic acid diagnosis kit for detecting schistosoma japonicum and detection method with nucleic acid diagnosis kit

Abstract

The invention provides a nucleic acid diagnosis kit for detecting schistosoma japonicum. The kit provides a group of primers which have high sensitivity and high specificity and are used for detectingschistosoma japonicum, wherein the primers have nucleic acid sequences as shown in SEQ ID NO.1-2. The invention constructs real-time fluorescent quantitative PCR detection technology with high sensitivity and high specificity for schistosoma japonicum free nucleic acids, and the detection technology can be used for accurately and quickly detecting infection of schistosoma japonicum, determining whether a water body contains miracidia and cercariae or not and performing species identification on schistosoma japonicum.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to the establishment of a Real-time PCR detection method for Schistosoma japonicum nucleic acid-specific gene G01 and its application in detecting free nucleic acid of Schistosoma japonicum. Background technique [0002] Schistosoma japonicum belongs to the class Trematodes, subclasses, orders, genus Schistosoma, and genus Schistosoma (called the genus Schizotysma in the veterinary system of our country). The final hosts of Schistosoma japonicum include more than 40 species of mammals such as humans, buffaloes, cattle, goats, sheep, pigs, horses, mules and dogs. Among them, sick domestic animals such as buffaloes, cattle, goats and sheep are the main sources of infection of Schistosoma japonicum. At present, in 74 countries and regions in the world, there are about 200 million people infected with schistosomiasis, of which 120 million people show clinical symptoms of schisto...

AI Technical Summary

Problems solved by technology

[0006] The key to nucleic acid diagnosis of Schistosoma japonicum is to find a nucleic acid fragment that stably exists in the host, but the number of target gene sequences used to amplify is limited. At present, the most commonly used target gene sequences are 18SrRNA gene and retrotransposon SjR2. , Sjrh1.0, mitochondrial DNA, etc., the specificity and sensitivity of these target sequences are still not ideal for on-site nucleic acid detection

The relatively mature methods for nucleic acid diagnosis include ordinary PCR and Real-time PGR. At present, more researches are on the highly sensitive LAMP method and recombinase amplification technology, but they are prone to false positives in on-site diagnosis. In addition, Recombinase amplification technology is relatively expensive

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