Method for detecting oxcarbazepine and 10, 11-dihydro-10-hydroxy carbamazepine in blood

A technology of hydroxycarbamazepine and dihydrogen, which is applied in the field of biological detection, can solve the problems of long detection time, low blood sample detection efficiency and long time, and achieve the effects of shortening detection time, accurate identification and short analysis time.

Inactive Publication Date: 2020-02-07
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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The detection time of blood samples is low due to the long ...

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  • Method for detecting oxcarbazepine and 10, 11-dihydro-10-hydroxy carbamazepine in blood
  • Method for detecting oxcarbazepine and 10, 11-dihydro-10-hydroxy carbamazepine in blood
  • Method for detecting oxcarbazepine and 10, 11-dihydro-10-hydroxy carbamazepine in blood

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[0041] In order to make the purpose, technical solution and advantages of the invention of the present application clearer, the technical solution of the present invention will be described below in conjunction with the accompanying drawings.

[0042] The method for detecting oxcarbazepine and 10 in blood of the present invention, 11-dihydro-10-hydroxyl carbamazepine comprises the following steps:

[0043] (1) Calibration of standard solution

[0044] (a) prepare the standard stock solution of oxcarbazepine and 10,11-dihydro-10-hydroxyl carbamazepine

[0045] Accurately weigh 1.71mg of oxcarbazepine standard substance and place it in a 2mL cryopreservation tube, dissolve it with pure methanol solution, and set the volume to 1mL to obtain a standard stock solution of oxcarbazepine, which is stored at -80°C;

[0046] Accurately weigh 2.87mg of 10,11-dihydro-10-hydroxycarbamazepine standard substance and place it in a 2mL cryopreservation tube, dissolve it with methanol solution...

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Abstract

The invention provides a method for detecting oxcarbazepine and 10, 11-dihydro-10-hydroxy carbamazepine in blood. The method comprises the following steps of preparing oxcarbazepine and 10, 11-dihydro-10-hydroxy-carbamazepine standard stock solution, preparing oxcarbazepine and 10, 11-dihydro-10-hydroxy-carbamazepine standard intermediate solution, preparing an internal standard working solution and preparing a standard solution, detecting the standard solution by utilizing a liquid chromatography-mass spectrometer, and fitting to obtain a standard curve equation corresponding to the oxcarbazepine and 10, 11-dihydro-10-hydroxy carbamazepine: y1=a*x1+b and y2=c*x2+d; treating the to-be-detected blood, detecting the to-be-detected blood by utilizing a liquid chromatography-mass spectrometer,and calculating the concentrations of oxcarbazepine and 10, 11-dihydro-10-hydroxy carbamazepine in the to-be-detected blood. The method disclosed by the invention is short in analysis time, small ininterference, quantitative and proper in internal standard, strong in specificity, and high in sensitivity.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for detecting oxcarbazepine and 10,11-dihydro-10-hydroxycarbamazepine in blood. Background technique [0002] Oxcarbazepine has a unique chemical structure and pharmacological effects. It is a mood stabilizer and is widely used clinically for localized and generalized epileptic seizures. 10,11-dihydro-10-hydroxycarbamazepine is the main active metabolite of oxcarbazepine, both of which have anticonvulsant activity, so the monitoring of oxcarbazepine and its active metabolite has important clinical significance. [0003] Currently, when blood samples from patients taking oxcarbazepine are tested, the test is usually performed using high-performance liquid chromatography. [0004] However, when the content of oxcarbazepine and its active metabolite 10,11-dihydro-10-hydroxycarbamazepine in the blood sample is detected by the above method, the blood sample need...

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Application Information

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IPC IPC(8): G01N30/89G01N30/04G01N30/72G01N30/86G01N30/06G01N30/14
CPCG01N30/04G01N30/06G01N30/14G01N30/72G01N30/8634G01N30/89G01N2030/042G01N2030/045
Inventor 雒琴贾永娟倪君君
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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