Method for detecting content of immunomarker, reagent and kit
A technology of markers and immune complexes, applied in the field of medical testing, can solve problems such as complex operations, long time-consuming, unstable results, etc., and achieve the effects of high detection sensitivity, good detection stability, and easy automation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0061] Example 1. Preparation and operation steps of heat shock protein 90α (Hsp90α) functionalized quantum dot-magnetic particle competitive immunoassay reagent
[0062] 1. Preparation method of fluorescent quantum dots for surface-modified Hsp90α antibody:
[0063] (1) Formation and purification of quantum dots-streptavidin (QDs-SA) conjugates
[0064] In PBS buffer (pH=8.5), 100 μL of QDs solution (20 μM) was mixed evenly with 500 μL of SA solution (80 μM). Then 100 μL of freshly prepared EDC solution (20 mg / mL) and 20 μL of freshly prepared NHS solution (20 mg / mL) were quickly added to the mixture, and incubated for 90 min at 25 °C with continuous shaking in the dark; In the filter unit (50,000MW), centrifuge at 10,000rpm at 4°C for 25 minutes, then apply to the chromatography column, pass through the membrane, centrifuge, wash 5 times with PBS (20mM, pH=8.5), and remove free non-conjugated SA by ultrafiltration and the isourea by-product of the conjugation reaction. Th...
Embodiment 2
[0083] Example 2. Preparation and stability comparison of carcinoembryonic antigen (CEA) functionalized quantum dot-magnetic particle competitive immunoassay reagent
[0084] 1. Preparation method of fluorescent quantum dots with surface-modified CEA antibody:
[0085] (1) Formation and purification of quantum dots-streptavidin (QDs-SA) conjugates
[0086] In Borate buffer (pH=8.5), 100 μL of QDs solution (20 μM) was mixed with 500 μL of SA solution (80 μM) evenly. Then 100 μL of freshly prepared EDC solution (20 mg / mL) and 20 μL of freshly prepared NHS solution (20 mg / mL) were quickly added to the mixture, and incubated for 90 min at 25 °C with continuous shaking in the dark; In the filter unit (50,000MW), centrifuge at 10,000rpm at 4°C for 25 minutes, then apply to the chromatography column, pass through the membrane, centrifuge, wash 5 times with Borate (20mM, pH=7.5), and remove free non-conjugated SA by ultrafiltration and the isourea by-product of the conjugation react...
Embodiment 3
[0106] Example 3. Preparation and recovery analysis of sugar chain antigen 72-4 (CA72-4) functionalized quantum dot-magnetic particle competitive immunoassay reagent
[0107] 1. Preparation method of fluorescent quantum dots for surface modification of CA72-4 antibody:
[0108] (1) Formation and purification of quantum dots-streptavidin (QDs-SA) conjugates
[0109] In PBS buffer (pH=7.4), 80 μL of QDs solution (20 μM) and 200 μL of SA solution (80 μM) were evenly mixed. Then 40 μL of freshly prepared EDC solution (20 mg / mL) and 20 μL of freshly prepared NHS solution (20 mg / mL) were quickly added to the mixture, and incubated at 25 °C for 90 min with continuous shaking in the dark; In the filter unit (50,000MW), centrifuge at 10000rpm at 4°C for 25 minutes, then apply to the chromatography column, pass through the membrane, centrifuge, wash 5 times with PBS (20mM, pH=7.4), and remove free non-conjugated SA by ultrafiltration and the isourea by-product of the conjugation react...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More