Application of osteopontin to hypoxic ischemic brain damage
A technology of hypoxia-ischemia and osteopontin, which is applied in the fields of biomedicine and molecular biology, and can solve problems such as unclear effects and underlying mechanisms
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[0085]1. Establishment and grouping of animal models: C57BL / 6J mice were purchased from the Experimental Animal Center of Shandong University after 15 days of pregnancy, and were raised under standardized environmental conditions. The animal research was approved by the Animal Ethics and Welfare Committee of Shandong University. On the 7th day after the birth of the mouse, the HI brain injury model was established. In short, the skin was cut along the middle right of the neck to expose and the right common carotid artery of the mouse was ligated, and the mouse was put back into the mouse cage to rest for 1 hour , and then the mice were placed in an anoxic box with 8% oxygen and 92% nitrogen for 1.5 hours.
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