Chromatographic strip comprising multiple test lines, diagnostic kit comprising same, and qualitative, semi-quantitative or quantitative analysis method comprising multiple competitive reaction measurement steps
A technology for diagnostic kits and chromatographic bands, applied in the field of chromatographic bands, can solve problems such as low detection limit and insufficient quantification
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0084] Embodiment 1: the making of chromatographic dipping (dip) band
[0085] A. Fabrication of test pads with test lines
[0086] Three test lines were rametted on nitrocellulose membranes. The nitrocellulose membrane was laminated on the plastic using a laminator. After that, rabbit IgG (rabbitimmunoglobulin G, Arista, USA) was used as the target substance or analogue of the test line, and an automatic ramming machine was used to divide the test line 1, test line 2, and test line 3 at regular intervals. After branching, drying was performed for 2 days (48 hours) at 25 to 30°C.
[0087] B. Absorbent pad (absorbance production of pad)
[0088] The absorbent pad is used without any treatment in a state where moisture is completely dried in a dryer, and is prepared by cutting it into an appropriate size.
[0089] C. Production of chromatographic bands
[0090] according to Figure 5 In the structure shown, the detection pad and the absorbent pad prepared through t...
Embodiment 2
[0097] Example 2: Analysis Using Dip Bands for Chromatography
[0098] 100 μl of a sample solution containing rabbit immunoglobulin (rabbitIgG) as a target substance and phosphate buffered saline (PBS) were divided into 96 well plates, respectively. The sample solution was prepared so that the concentration of rabbit immunoglobulin was 0, 5, 10, 50, 100, 500, 1000, and 5000 ug / ml, respectively. A labeling substance (fluorescence)-first ligand (anti-rabbit immunoglobulin) solution is added to the sample solution as a first predetermined amount.
[0099] The 96-well plate containing the above-mentioned sample solution was put into the chromatographic dipping tape prepared in the above-mentioned Example 1, and developed for 10 minutes.
[0100] Image 6It is a graph showing the development results of chromatographic bands based on the concentration of rabbit immunoglobulin as a target substance.
[0101] It was confirmed that for test line 1, test line 2, and test line 3, the ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com