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A culture method for improving the accumulation of cichoric acid in the adventitious roots of Echinacea purpurea in liquid culture

A culture method and liquid culture technology, applied in the biological field, can solve the problems of difficult market demand and low extraction rate, and achieve the effects of increasing content, simple operation and short production cycle

Active Publication Date: 2022-03-22
大连市现代农业生产发展服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chichoric acid has many uses, but the extraction rate from Echinacea purpurea or its adventitious root is low, so it is difficult to meet the market demand

Method used

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  • A culture method for improving the accumulation of cichoric acid in the adventitious roots of Echinacea purpurea in liquid culture
  • A culture method for improving the accumulation of cichoric acid in the adventitious roots of Echinacea purpurea in liquid culture
  • A culture method for improving the accumulation of cichoric acid in the adventitious roots of Echinacea purpurea in liquid culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) training

[0028] The adventitious roots of Echinacea purpurea cultivated for 480 hours were taken out under a sterile environment, shredded, and then placed in an air-lift bioreactor equipped with a liquid medium. The formula of the liquid medium is as follows: 3 / 4MS, 4wt% sucrose , pH 5.8, indolebutyric acid 1mg / L, air flow 1VVM, cultivated at 25±1°C, adjusted the pH to 4.5 after dissolving aspartame, sterilized at 123°C for 30min, and then added to the adventitious root cultured for 0 hours In the medium, the final concentrations of aspartame were adjusted to 50, 100, 150, and 200 mg / L, respectively. Harvest after culturing for 840 hours, take the adventitious roots without aspartame solution in the medium as the control (CK), and repeat each treatment 3 times.

[0029] (2) Content detection

[0030] Dry the harvested adventitious roots of Echinacea purpurea to constant weight, weigh 0.2g, add 20mL of methanol into a 50mL Erlenmeyer flask with a stopper, ultras...

Embodiment 2

[0037] (1) training

[0038] The adventitious roots of Echinacea angustifolia cultivated for 480 hours were taken out under a sterile environment, shredded, and then placed in a Erlenmeyer flask filled with a liquid medium. The formula of the liquid medium was as follows: 1 / 2MS, 3% sucrose, pH6.0, Indolebutyric acid 2mg / L, shake culture at 25±1°C at 80r / min. After dissolving aspartame, adjust the pH to 3.5, filter and sterilize through a 0.22 μm sterile filter membrane, and then add it to the medium for culturing adventitious roots for 240 hours, and adjust the final concentration of aspartame to 20 mg / L. Harvest after culturing for 840 hours, and take the adventitious roots without aspartame solution as the control, and each treatment was repeated 3 times.

[0039] (2) detection

[0040] The detection method is the same as in Example 1.

[0041] (3) Analysis of results

[0042] Chichoric acid concentrations were calculated and analyzed by paired t-test. It can be seen fr...

Embodiment 3

[0046] (1) training

[0047] The adventitious roots of Echinacea purpurea cultivated for 600 hours were taken out under a sterile environment, shredded, and placed in a Erlenmeyer flask equipped with a liquid medium. The liquid medium formula was as follows: 3 / 4MS, 4wt% sucrose, pH5.9, ind Indolebutyric acid 1mg / L, shake culture at 25±1°C at 80r / min. After dissolving aspartame, adjust the pH to 4.0, filter and sterilize through a 0.22 μm sterile filter membrane, and then add it to the adventitious root culture medium cultivated for 360 hours, and adjust the final concentration of aspartame to 100 mg / L. Harvest after culturing for 960 hours, and take the adventitious root without adding aspartame solution as a control.

[0048] (2) detection

[0049] The detection method is the same as in Example 1.

[0050] (3) Analysis of results

[0051] The analysis method is the same as in Example 2, and the results are shown in Table 3. As can be seen from Table 3, the cichoric acid co...

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Abstract

The invention relates to a cultivation method for increasing the accumulation of cichoric acid in adventitious roots of Echinacea purpurea, belonging to the field of biotechnology. The main technical scheme is as follows: a culture method for improving the accumulation of cichoric acid in adventitious roots of Echinacea purpurea, comprising the steps of: carrying out liquid culture of adventitious roots of Echinacea purpurea, adding sterile aspartame solution during 0-600 hours of cultivation, the aspartame The final concentration of the sweet solution is 20‑200mg / L. The aspartame solution is prepared by dissolving in deionized water, adjusting the pH of the solution to 3.5-4.5, and sterilizing. The method of the invention has the advantages of simple operation, aspartame with a concentration of 20-200 mg / L can significantly increase the content of cichoric acid in the adventitious root of Echinacea purpurea, short production cycle, low production cost, and is convenient for industrialized large-scale production and application of cichoric acid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a culture method for increasing the accumulation of cichoric acid in the adventitious roots of Echinacea purpurea in liquid culture. Background technique [0002] Echinacea spp is a perennial herb of the genus Echinacea in the Asteraceae family, native to North America. Echinacea is currently being developed as a drug mainly has three varieties, Echinacea purpurea purple, Echinacea angustifolia Echinacea angustifolia and Echinacea pallida white. Echinacea purpurea is a widely used medicinal plant and an internationally recognized immunomodulator, which has a significant effect on enhancing the body's immunity. The adventitious root culture of Echinacea purpurea to produce secondary products has become the focus of research in recent years. The adventitious roots of artificial suspension culture grow rapidly, the culture period is short, and the active ingredients are sta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008A01H4/005
Inventor 安冬吴春华王淼王媚孙丽娜赵彩云陈雪
Owner 大连市现代农业生产发展服务中心