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Washing liquid for staphylococcus aureus immunomagnetic beads

A technology of immune magnetic beads and staphylococci, which is applied in the field of immunity, can solve the problems of reducing the detection rate of target bacteria and loss of immune magnetic beads, and achieve the effect of improving cleaning function, reducing interference, and increasing detection rate

Pending Publication Date: 2020-02-21
GUANGDONG HUANKAI BIOLOGICAL SCI & TECH CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional immunomagnetic bead washing solution does not have a good effect in practical applications. When enriching complex food samples such as minced meat, a large amount of immunomagnetic beads will be lost, thereby reducing the detection rate of target bacteria.

Method used

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  • Washing liquid for staphylococcus aureus immunomagnetic beads
  • Washing liquid for staphylococcus aureus immunomagnetic beads
  • Washing liquid for staphylococcus aureus immunomagnetic beads

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1~8

[0039] The Staphylococcus aureus immune magnetic bead washing solution of Examples 1-8 was weighed according to the formulas 1-8 in Table 1, and the corresponding solution was prepared using PBS buffer (0.1M, PH7.4); mixed and stirred Mix well so that the pH of the washing solution is 7.0-7.5, and obtain the Staphylococcus aureus immune magnetic bead washing solution.

[0040]The conventional lotion formulation is a PBS (0.1M, PH7.4) solution containing 0.5g / L Tween-20.

[0041] Table 1 Different formulation components of immunomagnetic bead washing solution

[0042]

[0043]

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Abstract

The invention discloses a washing liquid for staphylococcus aureus immunomagnetic beads. Compared with a conventional washing liquid, through component optimization, the washing liquid has an improvedcleaning effect, effectively reduces the interference of a food matrix and has a specific and persistent bacteria increasing effect, and especially, the bacteria increasing effect on a sample with extremely low target bacteria content is remarkable, so that the detection rate of staphylococcus aureus is improved, and the application of the immunomagnetic beads in reagent detection is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of immunization, in particular to a Staphylococcus aureus immune magnetic bead washing solution. Background technique [0002] Staphylococcus aureus (Staphylococcus aureus) is one of the common food-borne pathogenic microorganisms, which can produce Staphylococcus aureus Enterotoxins (SEs) under appropriate conditions, which is the most important pathogenic factor. Staphylococcus aureus is widely distributed, and can be found in external environments such as air, water, humans and animals and their excreta; internal environments such as human and animal body surfaces, mucous membranes, respiratory tract, nasopharynx and intestinal tract are also distributed. 30%-50% of healthy people are carriers of Staphylococcus aureus, so Staphylococcus aureus is everywhere. [0003] In recent years, with the emergence of new detection technologies, especially molecular detection products, the sensitivity has been impro...

Claims

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Application Information

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IPC IPC(8): C12Q1/14
CPCC12Q1/14
Inventor 曲晓莹卢勉飞蔡芷荷滕昆仑吴清平陈博陈鲁
Owner GUANGDONG HUANKAI BIOLOGICAL SCI & TECH CO LTD
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