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Neutrophile granulocyte CD64 determination method for clinical infectious diseases

A technology for neutrophils and infectious diseases, applied in the field of neutrophils measurement, can solve the problems of inability to standardize and not be widely used in clinical practice, and achieve the effect of standardization

Pending Publication Date: 2020-02-21
徐州市儿童医院
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  • Application Information

AI Technical Summary

Problems solved by technology

The determination of CD64 is of great significance to the diagnosis of clinical infectious diseases, but because the determination of its fluorescence intensity is affected by factors such as instruments, reagents, and voltage, it cannot be standardized, and it has not been widely used clinically.

Method used

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  • Neutrophile granulocyte CD64 determination method for clinical infectious diseases
  • Neutrophile granulocyte CD64 determination method for clinical infectious diseases
  • Neutrophile granulocyte CD64 determination method for clinical infectious diseases

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] see Figure 1-Figure 3 As shown, the present invention provides a kind of technical scheme:

[0026] The present invention provides a method for measuring neutrophil CD64 for clinical infectious diseases, including the following steps:

[0027] S1. Sample collection: Collect 1-2 mL of fasting venous blood, anticoagulant with ethylenediaminetetraacetic acid;

[0028] S2. Flow cytometric detection: the sample is processed, and the processe...

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Abstract

The invention relates to the technical field of neutrophile granulocyte determination, in particular to a neutrophile granulocyte CD64 determination method for clinical infectious diseases. The methodcomprises the following steps of: collecting a sample; carrying out flow detection; and performing CD64 index calculation: the formula is as follows: CD64 index=(neutrophile granulocyte CD64 fluorescence intensity-lymphocyte CD64 fluorescence intensity) / (monocyte CD64 fluorescence intensity-lymphocyte CD64 fluorescence intensity)-(neutrophile granulocyte CD64 fluorescence intensity-lymphocyte CD64 fluorescence intensity). In the neutrophile granulocyte CD64 determination method for clinical infectious diseases, the fluorescence intensity of mononuclear cells is used as a positive control, andthe fluorescence intensity of lymphocytes is used as a negative control, so that the influence of factors such as instruments, reagents, voltage and the like on results can be eliminated, and the standardization of the results is facilitated.

Description

technical field [0001] The invention relates to the technical field of measuring neutrophils, in particular to a method for measuring neutrophil CD64 for clinical infectious diseases. Background technique [0002] CD64 is also known as Fc-gamma receptor protein 1 (FcyRI). CD64 shows low expression on the surface of neutrophils in peripheral blood. When the body is infected, bacterial cell wall lipopolysaccharide, granulocyte colony-stimulating factor, interferon and other stimulating factors can cause a rapid change from low expression to high expression of CD64 on the surface of neutrophils. , and activated neutrophils, so it was used as an infection indicator gate. The degree of activation caused by different pathogens is different, and the study found that it has a certain ability to distinguish between bacterial and viral infections. The measurement of CD64 is of great significance to the diagnosis of clinical infectious diseases, but because the measurement of its flu...

Claims

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Application Information

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IPC IPC(8): G01N15/14G01N21/64
CPCG01N15/1434G01N21/64
Inventor 方代华
Owner 徐州市儿童医院
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