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Application of prodigiosin in preparation of lymphangiomyomatosis cell proliferation inhibitors

A prodigiosin and cell proliferation technology, applied in the field of biomedicine, can solve the problem of unresearched Tsc2null inhibition of LAM disease cells

Inactive Publication Date: 2020-02-28
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the inhibitory effect of prodigiosin on the proliferation of Tsc2null cells in LAM disease has not been studied

Method used

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  • Application of prodigiosin in preparation of lymphangiomyomatosis cell proliferation inhibitors
  • Application of prodigiosin in preparation of lymphangiomyomatosis cell proliferation inhibitors
  • Application of prodigiosin in preparation of lymphangiomyomatosis cell proliferation inhibitors

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Embodiment 1

[0022] 1) Separation, purification and preparation of prodigiosin

[0023] Pick a ring of Serratia marcescens (S.marcescens) NJZT-1 from the glycerol storage tube, spread it on the LB solid medium for activation using the plate streak method, and cultivate it in a 26°C incubator for 3 days . A single colony of activated Serratia marcescens was picked and transferred to another solid medium, and cultured in a 26°C incubator for 3 days. The transfer was repeated 2-3 times to obtain a pure culture. A ring of culture was picked from the solid medium, inoculated into the liquid medium, and shaken at 26° C. and 180 r / min for 3 days. The culture solution was centrifuged to separate the supernatant and the precipitate; the lower layer of the precipitate was leached with methanol, centrifuged at 9000r / min for 10min, and the red pigment was separated. Use a rotary evaporator to rotary evaporate to obtain a deep purple pigment methanol solution. after C 18 Reverse-phase semi-prepara...

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Abstract

The invention discloses application of prodigiosin in preparation of lymphangiomyomatosis cell proliferation inhibitors, and relates to the field of biomedicine. The prodigiosin is obtained by a bioengineering technique, morphological changes of cells are observed through a microscope, changes of the cell activity are measured by a CCK-8 method, and the inhibitory effect of the prodigiosin on proliferation of lymphangiomyomatosis cells Tsc2null is observed. It is found that, within 24 hours, the inhibitory effect of the prodigiosin on the Tsc2null is obviously higher than on Tsc2wt; within 2 hours, the cell activity of the Tsc2null is inversely proportional to the concentration of prodigiosin; the higher the concentration of the prodigiosin is, the lower the cell activity is; the prodigiosin has a remarkable inhibitory effect on the proliferation of the lymphangiomyomatosis cells. Therefore, the prodigiosin is about to be widely applied to preparation of the lymphangiomyomatosis cell proliferation inhibitors, or drugs for resisting the proliferation of the lymphangiomyomatosis cells.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of prodigiosin in the preparation of lymphangioleiomyomatosis cell proliferation inhibitors. Background technique [0002] Lymphangioleiomyomatosis (LAM) is a rare disease characterized by diffuse pulmonary cystic lesions, which mainly occurs in women of childbearing age. The pathology is characterized by abnormal proliferation and infiltration of smooth muscle-like cells. The clinical manifestations of LAM are diverse. In addition to the characteristic cystic lesions in the lung, it can also involve many organs such as the kidney, lymphatic vessels and lymph nodes, spleen, and liver. Studies have shown that LAM is derived from TSC1 / TSC2 gene mutations in patients, among which TSC2 mutations are more common. TSC is a tumor suppressor gene. TSC-LAM patients are born with a TSC gene mutation at one site, and then acquire a second TSC gene mutation after birth to develop ...

Claims

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Application Information

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IPC IPC(8): A61K31/4025A61P35/00C07D207/44
CPCA61K31/4025A61P35/00C07D207/44
Inventor 赵银娟薛斌程琪沈紫竹
Owner NANJING FORESTRY UNIV