Utilization method of magnetic bead method based blood genome DNA extraction kit
A genome and kit technology, applied in the field of molecular biology, can solve the problems of increased extraction time, increased workload of experimenters, etc., and achieve the effect of safe operation process
Inactive Publication Date: 2020-02-28
上海睿璟生物科技有限公司
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Problems solved by technology
Although this method can quickly extract blood samples, as the number of samples increases, the extraction time will increase significantly due to the pretreatment steps of red blood cells, and the workload of experimenters will also increase accordingly.
Method used
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Embodiment Construction
[0014] The technical solution of the present invention will be further described below in conjunction with the embodiments.
[0015] A method for using the magnetic bead method blood genome DNA extraction kit of the present invention is characterized in that it comprises the following steps:
[0016] (1) Take 100 μL of blood sample into the sample tube, then add 300 μL of cell lysate, mix well, incubate at room temperature for 5 min, centrifuge at 12000 rpm for 1 min at room temperature, and remove the supernatant;
[0017] (2) Add 200 μL of lysate and 10 μL of proteinase K to the obtained precipitate in sequence, mix well and incubate for 15 min at a temperature of 56 °C and a rotation speed of 800 rpm;
[0018] (3) After standing at room temperature for 5-8 minutes, centrifuge at room temperature for 1-2 minutes at 10000-12000 rpm, transfer the supernatant to a new sample tube, add 100 μL of magnetic beads to the sample tube, and vortex with a vortex mixer Swirl and mix wel...
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The invention discloses a utilization method of a magnetic bead method based blood genome DNA extraction kit. The utilization method includes steps: taking 100MuL of a blood sample, adding 300MuL of cell lysis buffer, well mixing, performing room-temperature incubation for 5min, centrifuging for 1min at 12000rpm, and removing supernatant; adding 200MuL of lysis buffer and 10MuL of proteinase K, well mixing, and incubating for 15min under conditions of 56 DEG C and 800rpm; placing for 5min, centrifuging, transferring supernatant, adding 100MuL of magnetic beads, well mixing, incubating for 5min, centrifuging, and placing on a magnetic frame to enable solution clarification; after supernatant removal, adding ethanol solution, well mixing, placing on the magnetic frame, centrifuging to removethe ethanol solution, and standing until opacity of the magnetic beads is realized; adding 30MuL of nuclease-free water low-salt buffer, standing, placing on the magnetic frame for 2min, and transferring supernatant to a new sample tube to keep for standby application. The method has advantages that an extraction process does not involve toxic and harmful reagents, and safety in operation is realized.
Description
technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a method for using a magnetic bead method blood genome DNA extraction kit. Background technique [0002] In recent years, with the rapid development of molecular biology technology, the research on the genome level has become a hot spot for researchers. Many routine experiments in molecular biology need to extract genomic DNA as a prerequisite. Genomic DNA is the carrier of genetic information, including all genetic information including coding sequences and non-coding sequences. Extracting high-quality DNA from human or animal blood is an important prerequisite for genetic testing, precision medicine and other molecular biology research. [0003] At present, the commercial blood genomic DNA extraction method on the market is mainly the spin column method. The spin column type kit is easier to operate than the traditional DNA extraction method. The disadvantage is th...
Claims
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CPCC12N15/1013
Inventor 包文静金安娜高伙妮龚伟
Owner 上海睿璟生物科技有限公司