Method and kit for detecting aldosterone in blood plasma

A technology for detection kits and detection methods, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of ensuring accuracy, simple pre-treatment process, and small sample volume

Pending Publication Date: 2020-03-06
天津国科医疗科技发展有限公司
View PDF9 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the detection method and detection kit of aldosterone in plasma based on liquid chromatography-tandem mass spectrometry.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and kit for detecting aldosterone in blood plasma
  • Method and kit for detecting aldosterone in blood plasma
  • Method and kit for detecting aldosterone in blood plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0047] Unless otherwise defined, the technical terms used in the following embodiments have the same meaning as commonly understood by those skilled in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are conventional biochemical reagents; the experimental methods, unless otherwise specified, are conventional methods.

[0048] The present invention will be described in detail below in conjunction with the embodiments and accompanying drawings.

[0049] 1. Reagent source

[0050] (1) Aldosterone (Ald): Shanghai Pufen Bio, CAS No. 52-39-1, is a dry powder reagent, 1mg / bottle, reconstituted with methanol before use.

[0051] (2) Aldosterone-d 7 (Ald–d 7): Shanghai Pufen Biology, CAS No. 1261254-31-2, liquid reagent, 100 μg / mL, reconstituted with methanol before use.

[0052] (3) Methanol: Reagent No. 67-56-1 from Fisher Chemical Company was used, the purity was LC / MS grade, and it was sealed and stor...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method and kit for detecting aldosterone in blood plasma. The kit for detecting aldosterone in blood plasma comprises a calibration product and a quality control product. Thecalibration product and the quality control product are bovine serum albumin containing aldosterone. According to the kit for detecting aldosterone in blood plasma, which is provided by the invention,the calibration product, a reagent involved in sample pretreatment and the quality control product are provided; tedious steps and errors caused by laboratory detection and self-preparation are avoided; and the accuracy of a detection result is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of clinical in vitro detection reagents, and in particular relates to a detection method and a detection kit for aldosterone in blood plasma, which can be used for detection of aldosterone in blood plasma. Background technique [0002] There are two types of hypertension, primary hypertension and secondary hypertension. The probability of cardiovascular events in secondary hypertension is higher than that in primary hypertension. Primary aldosteronism (PA) is the most common cause of secondary hypertension, and almost 10%. If diagnosed early, secondary hypertension can be treated with surgery or drugs to bring blood pressure back to normal levels, improve the prognosis of patients, and greatly reduce the incidence of cardiovascular diseases. [0003] Aldosterone is a mineralocorticoid secreted by the zona glomerulus of the adrenal cortex. Its main physiological function is to maintain sodium and excrete p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/86G01N30/88
CPCG01N30/06G01N30/8624G01N30/88G01N2030/062G01N2030/8648G01N2030/8813
Inventor 李艳周传贵周玉松胡玮徐振程文播
Owner 天津国科医疗科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap