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Tumor marker serum cold-inducible RNA-binding protein for liver cancer and application thereof

A technology of tumor markers and binding proteins, which is applied in the field of liver cancer tumor markers serum cold-inducible RNA binding proteins, can solve the problems of no research publications and achieve significant clinical application prospects

Pending Publication Date: 2020-03-13
THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, serum CIRP is an important inflammatory mediator after liver function damage, but no research has been published on its application in liver cancer

Method used

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  • Tumor marker serum cold-inducible RNA-binding protein for liver cancer and application thereof
  • Tumor marker serum cold-inducible RNA-binding protein for liver cancer and application thereof
  • Tumor marker serum cold-inducible RNA-binding protein for liver cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Detection of CIRP expression and secretion in the serum of non-tumor patients and liver cancer patients

[0028] 1. Collection of clinical serum samples

[0029] Materials: Serum from non-tumor patients (including patients with simple liver cirrhosis and normal adult volunteers) and liver cancer patients were collected from the First Affiliated Hospital of Xi'an Jiaotong University. Sera from 173 patients diagnosed with liver cancer between 2012 and 2016 were collected. The diagnosis of liver cancer patients was obtained by enhanced CT scan, angiography and histopathological examination. At the same time, 124 healthy volunteers without liver disease history, 71 patients with liver cirrhosis and 39 patients with chronic hepatitis were obtained. The entire collection and experimental testing process was certified by the Ethics Committee of the First Affiliated Hospital of Xi'an Jiaotong University. Informed consent was signed for all serum samples before col...

Embodiment 2

[0057] Example 2: Expression of serum CIRP after orthotopic tumor formation of mouse liver cancer cell line H22 in C57 mice

[0058] 1. H22 cell intraperitoneal inoculation

[0059] 1. After the cryopreserved mouse liver cancer H22 cells were harvested, they were continuously shaken in warm water at 37°C to melt the cells rapidly;

[0060] 2. Centrifuge at low speed for 5 minutes, discard the supernatant;

[0061] 3. Use sterile saline to resuspend the cells and adjust the concentration to 1×10 7 / ml;

[0062] 4. The abdomen of the mouse was fully disinfected with iodophor, and 0.2ml of cell suspension was injected intraperitoneally;

[0063] 5. Seven days after the injection, ascitic fluid was extracted for cell culture and passage.

[0064] 6. Use Luciferase to transfect the third-generation cells;

[0065] 7. Aseptically obtain the transfected H22 cells for subsequent liver injection.

[0066] 2. Construction of orthotopic tumorigenesis model of mouse liver cancer

...

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Abstract

The invention relates to the field of early diagnosis of tumors and biomedicine, in particular to application of a serum cold-inducible RNA-binding protein (CIRP) as a tumor marker for liver cancer. The invention describes the application of a concentration detection reagent for the serum CIRP in preparing an early tumor diagnosis or prognosis detection reagent for liver cancer. According to the invention, the increased CIRP secretion in the serum identified by the standard ELISA method can be used as a diagnostic marker for early liver cancer, can be used for evaluation of prognosis, and hasa significant clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to early diagnosis of tumors, in particular to a liver cancer tumor marker serum cold-inducible RNA binding protein and application thereof. Background technique [0002] Primary liver cancer (hepatocellular carcinoma, HCC) is one of the malignant tumors with high morbidity and mortality in the world, which seriously endangers human health. In recent years, with the increase in the number of people with chronic viral hepatitis and non-alcoholic fatty liver, their incidence has gradually increased. According to the World Health Organization's International Cancer Center, there are about 800,000 new cases of liver cancer each year, 80% of which occur in developing countries, and China accounts for 55%. Its death rate ranks second among malignant tumors in my country. bit. Although the curative effect has been improved in the past two decades with the advancement of diagnostic techni...

Claims

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Application Information

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IPC IPC(8): C07K14/47G01N33/68G01N33/574
CPCC07K14/47G01N33/68G01N33/57438G01N33/57484
Inventor 吴荣谦杜肇清柯梦云吕毅
Owner THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
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