circRNA marker associated with early diagnosis of colorectal cancer, detection primer, reagent kit and application of circRNA marker

A technology for early diagnosis and colorectal cancer, applied in the fields of genetic engineering and oncology, it can solve the problem of little knowledge of circRNA, and achieve the effect of inhibiting growth and metastasis, accurate quantification, and easy to use.

Pending Publication Date: 2020-03-20
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, little is known about circRNAs a

Method used

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  • circRNA marker associated with early diagnosis of colorectal cancer, detection primer, reagent kit and application of circRNA marker
  • circRNA marker associated with early diagnosis of colorectal cancer, detection primer, reagent kit and application of circRNA marker
  • circRNA marker associated with early diagnosis of colorectal cancer, detection primer, reagent kit and application of circRNA marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] The circular RNA marker related to the early diagnosis of colorectal cancer in this example is has-circ-007667, which has the nucleotide sequence shown in SEQ ID NO.3.

Embodiment 2

[0094] The primers used in this example to detect the expression level of circular RNA markers related to the early diagnosis of colorectal cancer were designed and synthesized according to the sequence of has-circ-007667, and the primer sequences are as follows:

[0095] has-circ-007667 Primer F (SEQ ID NO.1): 5'-AGAGCCCTCTGAGGAAAGCATC-3';

[0096] has-circ-007667 Primer R (SEQ ID NO. 2): 5'-GTACTGGTGCAGGCTCCAA-3'.

[0097] The above primers can be used to detect the expression level of has-circ-007667 in the sample, which can be used as effective information for early diagnosis of colorectal cancer.

Embodiment 3

[0099] The circRNA kit (for 30 reactions) used for the early diagnosis of colorectal cancer in this embodiment is composed as follows:

[0100]

[0101] Using the kit of this example, combined with commonly used RNA extraction reagents and general reverse transcription reagents, can specifically detect the expression level of has-circ-007667 in tissues, and can be effectively used for early auxiliary diagnosis of colorectal cancer.

[0102] Check the effect of the circRNA kit in this example:

[0103] 50 cases of colorectal cancer from the Department of Gastrointestinal Surgery of the First Affiliated Hospital of Xinxiang Medical College were selected from 2018 to 2019, total RNA was extracted from fresh tissue samples, and the relative expression of has-circ-007667 was detected by the method of Test Example 3.

[0104] The results showed that the circRNA kit can specifically and effectively amplify the has-circ-007667 sequence. The results of statistical analysis showed t...

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Abstract

The invention discloses a circRNA marker associated with early diagnosis of colorectal cancer, a detection primer, a reagent kit and an application of the circRNA marker, and belongs to the fields ofgenetic engineering and oncology. The circRNA marker associated with early diagnosis of colorectal cancer is has-circ-007667, and circRNA shows specific low expression in colorectal cancer tissues, and does not show such specificity low expression in normal tissue, so that the has-circ-007667 can be used as a specific molecular marker to improve the diagnosis efficiency of the colorectal cancer, and can also be used for early assistance diagnosis of clinical colorectal cancer. The specific detection primer synthesized according to a has-circ-007667 sequence can be used for detecting the expression level of the has-circ-007667 in samples, so that the sensibility and the specificity for clinical colorectal cancer early diagnosis can be improved, and the detection results are accurate and stable.

Description

technical field [0001] The invention relates to a circular RNA marker related to the early diagnosis of colorectal cancer, detection primers, kits and applications designed for the circular RNA marker, belonging to the fields of genetic engineering and oncology. Background technique [0002] Circular RNA (circRNA) is a type of endogenous non-coding RNA molecule produced by reverse shearing force, which is a covalently closed circular structure without a 5' end cap and a 3' end poly(A) tail. Due to the special structure of circular RNA, it has higher resistance to nucleases, is more stable than linear RNA, and is not easy to degrade. It can exist in large numbers and stably in the cytoplasm of eukaryotes. Its abundance is sometimes Even more than 10 times higher than the corresponding linear mRNA. Circular RNAs play the role of miRNA sponges in different species, called competitive endogenous RNAs (ceRNAs), which can competitively bind miRNAs to regulate the expression of ta...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 王芳王天云董卫华苗馨之房祥杰林艳米春柳樊振林王蒙段树燕
Owner XINXIANG MEDICAL UNIV
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