Purpose of resveratrol and in vitro activation primordial follicle reagent and method
A technology of resveratrol and primordial follicles, applied in biochemical equipment and methods, reproductive cells, microorganisms, etc., to reduce pain, shorten activation time, and improve efficiency
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Embodiment 1
[0041] Experimental Materials:
[0042]5-day-old C57BL / 6J female mice and 35-day-old C57BL / 6J female mice, 20 each. 20 pieces of human ovarian tissue.
[0043] Experimental reagents:
[0044] Reagent A: Ovarian Basal Medium
[0045] Reagent B: 40mM Resveratrol
[0046] Reagent C: sterile PBS
[0047] experimental method
[0048] 1. Add 1.2 mL of reagent A and 1.2 μL of reagent B to a 6-well plate, and set up a blank control with only reagent A added. Each group of reagents were preheated at 37°C for 10 minutes;
[0049] 2. Cut the corresponding ovarian cortex tissue into 1mm in Reagent C 3 Put the small pieces on the above-mentioned preheated 6-well plate, and then put them into the cell incubator for 30 minutes. The culture conditions are 37°C, 5% CO 2 .
[0050] 3. After step 2, after washing with sterile PBS, the tissues of each group were further cultured with reagent A for 12 hours, and the levels of SIRT1, phosphorylated mTOR, AKT, and total mTOR and AKT levels ...
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