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Purpose of resveratrol and in vitro activation primordial follicle reagent and method

A technology of resveratrol and primordial follicles, applied in biochemical equipment and methods, reproductive cells, microorganisms, etc., to reduce pain, shorten activation time, and improve efficiency

Inactive Publication Date: 2020-03-27
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies in recent years have shown that resveratrol has a wide range of biological activities, but there is no information about the effect of resveratrol on the activation of primordial follicles in vitro

Method used

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  • Purpose of resveratrol and in vitro activation primordial follicle reagent and method
  • Purpose of resveratrol and in vitro activation primordial follicle reagent and method
  • Purpose of resveratrol and in vitro activation primordial follicle reagent and method

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Embodiment 1

[0041] Experimental Materials:

[0042]5-day-old C57BL / 6J female mice and 35-day-old C57BL / 6J female mice, 20 each. 20 pieces of human ovarian tissue.

[0043] Experimental reagents:

[0044] Reagent A: Ovarian Basal Medium

[0045] Reagent B: 40mM Resveratrol

[0046] Reagent C: sterile PBS

[0047] experimental method

[0048] 1. Add 1.2 mL of reagent A and 1.2 μL of reagent B to a 6-well plate, and set up a blank control with only reagent A added. Each group of reagents were preheated at 37°C for 10 minutes;

[0049] 2. Cut the corresponding ovarian cortex tissue into 1mm in Reagent C 3 Put the small pieces on the above-mentioned preheated 6-well plate, and then put them into the cell incubator for 30 minutes. The culture conditions are 37°C, 5% CO 2 .

[0050] 3. After step 2, after washing with sterile PBS, the tissues of each group were further cultured with reagent A for 12 hours, and the levels of SIRT1, phosphorylated mTOR, AKT, and total mTOR and AKT levels ...

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Abstract

The invention relates to the technical field of biology, in particular to purpose of resveratrol and an in vitro activation primordial follicle reagent and method. Resveratrol is used for treating ovary cortex tissue, and besides, activating key protein of two signal channels of AKT and mTOR, and also the effect of promoting the SIRT1 level. When the ovary cortex tissue is cultured in an activating culture solution containing resveratrol, dormant primordial follicles can be awakened in vitro, and then surgerical transplantation can be performed. The resveratrol is a natural substance which isrich in human foods of wine and the like, and toxic and side effects of the resveratrol on organisms are not reported, so that feasibility and safety of the resveratrol for IVA are worth affirmation and are even better than those of conventional IVA medicines. Besides, the resveratrol can improve the efficiency when the primordial follicles are applied to IVA, namely that in vitro activation timecan be shortened to 30 minutes, and besides, a patient only needs a single-time surgery, so that pain, surgery fees and the like of the patient can be greatly reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the use of resveratrol and an in vitro activation reagent and method for primordial follicles. Background technique [0002] In female mammals, the follicle is the basic functional unit of the ovary. The mammalian follicle is composed of oocytes located in the center of the follicle, which are arrested in the first meiotic prophase, granulosa cells wrapped around it, and the final stage. The outer layer is composed of membranous cells. The primordial follicle bank of mammals is established before and after birth. Since then, the number of primordial follicles will not increase and will decrease periodically with age, so primordial follicles are a non-renewable resource. [0003] After the primordial follicle bank is established, less than 5% of the primordial follicles can be activated and enter the stage of growing follicles each time, while most of the primordial follicles in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2500/35
Inventor 夏国良王超张拓何美娜杜新华赵立华
Owner CHINA AGRI UNIV
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