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A local anti-tumor fiber implant containing Bifidobacterium bifidum and its preparation method and application

A bifidobacteria bifidum and antitumor technology, applied in the field of electrospinning, can solve the problems of difficult preservation of bifidobacteria bifidum activity, and achieve the effects of good water solubility, preventing bacterial aggregation, and prolonging the preservation time.

Active Publication Date: 2021-11-30
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although it has been reported to load bacteria into electrospun fiber membranes, it has not been reported to load Bifidobacterium bifidum into core-shell electrospun fiber membranes in which both phases are aqueous. Activity is difficult to preserve

Method used

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  • A local anti-tumor fiber implant containing Bifidobacterium bifidum and its preparation method and application
  • A local anti-tumor fiber implant containing Bifidobacterium bifidum and its preparation method and application
  • A local anti-tumor fiber implant containing Bifidobacterium bifidum and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0066] This embodiment provides a preparation method of an anti-tumor fiber local implant containing Bifidobacterium bifidum, which specifically includes the following steps, and the model of the instrument used is: electrospinning machine (KH.08, Beijing Concent Technology company); Microflow pump (LSP02.1B, LongerPump);

[0067] (1) Bifidobacterium bifidum strains were purchased from China Industrial Microorganism Culture Collection Management Center, (CICC) No. 10395, and Bifidobacterium bifidum was anaerobically cultured to logarithmic growth phase (OD value 0.6-0.8), The culture medium is MRS broth medium, centrifuged at 4000g for 10min to collect the cells, washed repeatedly with phosphate buffered saline solution 3 times, and added 1mL of 10% (w / v) skimmed milk to the washed cell pellet to resuspend for use .

[0068] (2) Dissolve 1 g of polyvinyl alcohol (PVA) in 10 mL of deionized water, and stir in a water bath at 80° C. until all the polyvinyl alcohol is dissolved ...

Embodiment 2

[0074] Carboxyfluorescein diacetate is a hydrophobic dye, which can enter the cell through Bifidobacterium bifidum with complete cell membrane, and generate carboxyfluorescein through intracellular non-specific esterase hydrolysis, which can make Bifidobacterium bifidum present the whole Cells are stained green.

[0075] This embodiment provides a preparation method of an anti-tumor fiber local implant containing Bifidobacterium bifidum, which specifically includes the following steps:

[0076] (1) Take Bifidobacterium bifidum and stain with 5mM carboxyfluorescein diacetate (5-CFDA) for 30min, centrifuge the bacteria at 4000g for 10min, collect the bacterial precipitate, wash repeatedly three times with phosphate buffer solution (pH 7.4), discard residual dye. Add deionized water to resuspend, evaporate to dry, and observe under a fluorescence microscope (Axio Observer, ZEISS). figure 1 As shown, Bifidobacterium bifidum stained green can be observed.

[0077] (2) Bifidobact...

Embodiment 3

[0080] Bifidobacterium bifidum and 10% polyvinyl alcohol spinning solution were mixed and placed in the inner tube of the coaxial needle, and 35% gelatin / genipin (the mass ratio of gelatin and genipin was 10:1) solution was placed in the same The outer tube of the shaft needle is electrospun with high-voltage static electricity, and the specific operation process and parameters are the same as in Example 1, except that the flow rate of the inner and outer phases is increased to 2.5mL / h, and the collected fiber membrane is placed in a vacuum Fully dry in a drying oven and observe with a scanning electron microscope.

[0081] image 3 An electron micrograph of Bifidobacterium bifidum under a scanning microscope. The cell wall of Bifidobacterium bifidum is irregular, uneven, enlarged or agglomerated, with one or more branches. In the field of view with great difference in length, round spheres can be seen, rod-shaped and bifurcated reversible Sexual variation, which is a phenot...

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Abstract

The invention discloses an anti-tumor fiber local implant containing Bifidobacterium bifidum and its preparation method and application, and relates to the technical field of electrospinning, which comprises the following steps: passing the inner phase spinning solution into a coaxial needle The inner tube of the invention, wherein, the inner phase spinning liquid contains Bifidobacterium bifidum; the outer phase spinning liquid is passed into the outer tube of the coaxial needle for electrospinning, and the electrospun electrospinning method with the inner phase coated with Bifidobacterium bifidum is obtained. Fiber membrane. The preparation method can well coat the bifidobacterium bifidum in the inner core of the electrospun fiber membrane through the coaxial electrospinning technology, thereby protecting the activity of the bifidobacterium bifidum. The materials of the external spinning solution and the internal spinning solution have high oxygen resistance, thereby maintaining the activity of Bifidobacterium bifidum.

Description

technical field [0001] The invention relates to the technical field of electrospinning, in particular to an anti-tumor fiber local implant containing Bifidobacterium bifidum and its preparation method and application. Background technique [0002] At present, bacterial therapy has many unique advantages as an emerging cancer treatment. [0003] Zheng et al. (2018) used bacterial tumor targeting to target carbon dots C 3 N 4 Electrostatic combination with Escherichia coli MG1655 with its own NO synthase is used for tumor treatment. Under the light greater than 630nm, the photoelectrons generated by carbon dots can trigger the nitrate reduction reaction in Escherichia coli to generate NO, and the tumor inhibition rate reaches 80%. Fan et al. (2018) used Escherichia coli modified with a heat-sensitive promoter and a therapeutic protein gene TNF-α combined with gold nanoparticles (AuNP) to anaerobically target homing tumors for therapeutic effect. [0004] Most of the bacteri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/745A61K9/00A61K47/36A61K47/42A61K47/32A61P35/00D04H1/728D01D5/00
CPCA61K9/0024A61K35/745A61K47/32A61K47/36A61K47/42A61P35/00D01D5/0038D01D5/34D04H1/728
Inventor 周绍兵徐玲杨光杜天意李西林何洋李艳
Owner SOUTHWEST JIAOTONG UNIV
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