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A plant two-step fermentation product with skin anti-irritation and anti-oxidation effects and its preparation method and application

A two-step fermentation and anti-irritation technology, which is applied in skin care preparations, medical preparations containing active ingredients, cosmetic preparations, etc., can solve the problem of high cost of raw materials and time, heat-labile active ingredients, damage to medicinal materials, etc. problems, to achieve the effect of improving the bioavailability of phenols, increasing the antioxidant activity, and changing the water solubility

Active Publication Date: 2022-04-15
肽源(广州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fermentation process introduced in this invention patent improves the skin repairing effect of Chinese herbal medicine raw materials, but there are some shortcomings: on the one hand, due to the demanding nutritional requirements of medicinal fungi and the long production cycle of solid-state fermentation, the cost of raw materials for this process and time costs are high; on the other hand, because medicinal fungi are easy to infect bacteria during the fermentation process, it is usually required to sterilize the culture substrate at high temperature, which will destroy the heat-labile active ingredients in the medicinal materials

Method used

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  • A plant two-step fermentation product with skin anti-irritation and anti-oxidation effects and its preparation method and application
  • A plant two-step fermentation product with skin anti-irritation and anti-oxidation effects and its preparation method and application
  • A plant two-step fermentation product with skin anti-irritation and anti-oxidation effects and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0040] This embodiment is the preparation of honeysuckle two-step fermentation product:

[0041] (1) Preparation of plant culture medium: add 120g of honeysuckle dry powder, 60g of glucose, 30g of fructooligosaccharides, 3g of nisin, and 6L of water into a 10L fermenter, mix well; incubate at 90°C for 15min for disinfection, then cool to 36°C;

[0042] (2) Anaerobic fermentation of lactic acid bacteria: use 240mL MRS broth medium shake flask to cultivate Lactobacillus plantarum in advance, and inoculate the bacterial solution into the plant medium prepared in step (1) under aseptic operation, the inoculation amount of the bacterial solution is 4 %, keep the temperature at 36°C, dissolve oxygen at 0-50%, stir at 150rpm, and cultivate for 18h.

[0043] (3) Saccharomyces aerobic fermentation: use 150mL 4% wort medium shake flask to culture S. %, and 60mL 0.1g / mL silk protein peptide aqueous solution was added to the above-mentioned fermentation broth, the temperature was mainta...

Embodiment 2

[0049] This embodiment is the preparation of purslane two-step fermentation product:

[0050] (1) Preparation of plant culture medium: add 120g of purslane dry powder, 60g of glucose, 30g of fructooligosaccharides, 3g of nisin, and 6L of water into a 10L fermenter, and mix well; incubate at 90°C for 15min for disinfection, then Cool to 36°C.

[0051] (2) Anaerobic fermentation of lactic acid bacteria: use 240mL MRS broth medium shake flask to cultivate Lactobacillus plantarum in advance, and inoculate the bacterial liquid into the plant medium prepared in step (1) under aseptic operation, the inoculation amount of the bacterial liquid is 6 %, keep the temperature at 36°C, dissolve oxygen at 0-50%, stir at 150rpm, and cultivate for 24h.

[0052] (3) Saccharomyces aerobic fermentation: use 150mL 4% wort medium to shake the flask to cultivate S. %, and 60mL 0.1g / mL silk protein peptide aqueous solution was added to the above-mentioned fermentation broth, the temperature was kep...

Embodiment 3

[0058] This embodiment is the preparation of purslane two-step fermentation product:

[0059] (1) Preparation of plant culture medium: add 120g of purslane dry powder, 60g of glucose, 30g of fructooligosaccharides, 3g of nisin, and 6L of water into a 10L fermenter, and mix well; incubate at 90°C for 15min for disinfection, then Cool to 36°C.

[0060] (2) Anaerobic fermentation of lactic acid bacteria: use 240mL MRS broth medium shake flask to cultivate Lactobacillus plantarum in advance, and inoculate the bacterial liquid into the plant medium prepared in step (1) under aseptic operation, the inoculation amount of the bacterial liquid is 6 %, keep the temperature at 36°C, dissolve oxygen at 0-50%, stir at 150rpm, and cultivate for 24h.

[0061] (3) Saccharomyces aerobic fermentation: use 150mL 4% wort medium to shake the flask to cultivate S. %, keep the temperature at 28°C, dissolve oxygen at 70-90%, stir at 250rpm, and incubate for 3h.

[0062] (4) Cell lysis: adjust the ...

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Abstract

The invention belongs to the technical field of cosmetic raw materials, and discloses a plant two-step fermentation product with skin anti-irritation and anti-oxidation effects, a preparation method and application thereof. The preparation method comprises the following operation steps: using lactic acid bacteria to carry out anaerobic liquid fermentation on the plant culture medium; the formula of the plant culture medium is: plant dry powder with a mass fraction of 2-5%, glucose with a mass fraction of 1%-3%, and a mass fraction of 0-3%. 1% fructo-oligosaccharides, 0.05-0.1% nisin by mass fraction, and the balance is water; the dry plant powder is more than one dry powder of honeysuckle, purslane, pomegranate, mugwort and chamomile; Saccharomyces cerevisiae Perform aerobic liquid fermentation on anaerobic liquid fermentation products; lyse, filter, and centrifuge aerobic liquid fermentation products to obtain clarified or semi-clear liquid plant second-step fermentation products.

Description

technical field [0001] The invention belongs to the technical field of cosmetic raw materials, and in particular relates to a plant two-step fermentation product with skin anti-irritation and anti-oxidation effects, a preparation method and application thereof. Background technique [0002] There are many types of plants that have anti-irritant and anti-oxidative effects on the skin, such as honeysuckle, purslane, pomegranate, grape, mugwort, chamomile, etc. These plants usually contain polyphenolic compounds. However, polyphenolic compounds have certain cytotoxicity while having efficacy, which limits the dosage and scope of application of plant extracts. The main active ingredients flavonoids in these functional plants have poor water solubility, which also limits the bioavailability of plant active ingredients. [0003] Natural active ingredients have become a hot spot in the cosmetics market, especially in the skin care market. The market focus started from natural pla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61Q19/08A61Q19/00A61Q19/02
CPCA61K8/9789A61Q19/08A61Q19/005A61Q19/02A61K2800/85
Inventor 黄亚东梁嘉亮张齐肖巧学
Owner 肽源(广州)生物科技有限公司
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