Tissue culture rapid propagation method of acer elegantulum
A tissue culture rapid propagation and beautiful technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of slow reproduction speed and large differences in morphological characteristics, so as to solve the problems of slow reproduction speed and difference in morphological characteristics. larger effect
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Embodiment 1
[0016] A method for tissue culture and rapid propagation of Acer elegans, the specific steps are as follows:
[0017] (1) collect robust, no damage by diseases and insect pests, good growth branches, cut into 3-5cm sections, as explants;
[0018] (2) Disinfect the explant: rinse with clear water for 3-5min, then soak with 75% ethanol for 15-20s, rinse with clear water; then soak with 5% sodium hypochlorite solution for 30s;
[0019] (3) Inoculate the sterilized explants into WPM+1mg / L 6-BA+0.3mg / L IAA+10g / L sucrose+4g / L agar medium, the pH of the medium is 5.6-5.9; 15°C Cultivate under conditions for 15 days, and the light intensity is 2000lx, forming adventitious buds;
[0020] (4) the adventitious sprouts that step (3) obtains in WPM+0.1mg / L NAA+1mg / L IBA+1mg / L GA 3 + 15g / L sucrose + 4g / L agar medium for culture, the pH of the medium is 5.4-5.8; culture in total darkness at 25°C for 5 days, and then place it under the condition of 12 hours of light every day, and the light...
Embodiment 2
[0024] A method for tissue culture and rapid propagation of Acer elegans, the specific steps are as follows:
[0025] (3) collect robust, no damage by disease and insect, the branch with good growth condition, be cut into the section of 3-5cm, as explant;
[0026] (4) Disinfect the explant: rinse with clear water for 3-5min, then soak with 75% ethanol for 15-20s, rinse with clear water; then soak with 8% sodium hypochlorite solution for 25s;
[0027] (3) Inoculate the sterilized explants into WPM+1.5mg / L 6-BA+0.4mg / L IAA+12g / L sucrose+5g / L agar medium, and the pH of the medium is 5.6-5.9; 15 Cultivate at -18°C for 13 days with a light intensity of 2500lx to form adventitious buds;
[0028] (4) the adventitious sprouts that step (3) obtains in WPM+0.2mg / L NAA+1.5mg / L IBA+1.5mg / L GA 3 + 18g / L sucrose + 5g / L agar medium for culture, the pH of the medium is 5.4-5.8; culture in total darkness at 28°C for 3 days, and then place it under the condition of 12 hours of light every day...
Embodiment 3
[0032] A method for tissue culture and rapid propagation of Acer elegans, the specific steps are as follows:
[0033] (5) collect robust, no damage by disease and insect, the branch with good growth condition, be cut into the section of 3-5cm, as explant;
[0034] (6) Disinfect the explant: rinse with clear water for 3-5min, then soak with 75% ethanol for 15-20s, rinse with clear water; then soak with 10% sodium hypochlorite solution for 20s;
[0035] (3) Inoculate the sterilized explants into WPM+3mg / L 6-BA+0.5mg / L IAA+15g / L sucrose+6g / L agar medium, the pH of the medium is 5.6-5.9; 18°C Cultivate under the conditions for 10 days, and the light intensity is 3000lx, forming adventitious buds;
[0036] (4) the adventitious sprouts that step (3) obtains in WPM+0.3mg / L NAA+2mg / L IBA+2mg / L GA 3 + 20g / L sucrose + 6g / L agar medium for culture, the pH of the medium is 5.4-5.8; culture in total darkness at 28°C for 3 days, and then place it under the condition of 12 hours of light e...
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