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Gene sequence sorting synthesizing device and sorting synthesizing method

A technology for gene sequence and synthesis devices, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problems of complex machines, low throughput, inflexibility, etc. , The effect of high synthesis throughput and cost reduction

Active Publication Date: 2020-04-17
GUANGDONG SHUNDE IND DESIGN INST GUANGDONG SHUNDE INNOVATIVE DESIGN INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the base pair quantity of most nucleic acids that people need far exceeds the base pair quantity of the longest nucleic acid chain that current DNA synthesizer can synthesize, the DNA synthesizer in the prior art mainly has the following problems, (1) generally (2) The synthesis column and the carrier need to be manually installed, and the operation is complicated; (3) The amplification of the synthesis column is limited, inflexible, and the synthesis cost is high; (4) The machine is complicated, the failure rate is high, high cost

Method used

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  • Gene sequence sorting synthesizing device and sorting synthesizing method
  • Gene sequence sorting synthesizing device and sorting synthesizing method
  • Gene sequence sorting synthesizing device and sorting synthesizing method

Examples

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Embodiment 1

[0038] see figure 1 As shown, this embodiment provides a gene sequence sorting synthesis device 10, which includes a first reactor 100, a second reactor 200, a first sorter 310, a second sorter 320, a first sorter pipeline 410 and the second sorting pipeline 420 .

[0039]The first reactor 100 includes several first reaction pipelines 110 arranged side by side, the first reaction pipeline 110 has a first reaction channel, and the size of the first reaction channel is greater than the size of the glass beads and less than twice the size of the glass beads.

[0040] The second reactor 200 includes several second reaction pipelines 210 arranged in parallel. The second reaction pipeline 210 has a second reaction channel, the size of the second reaction channel is greater than the size of the glass bead and less than twice the size of the glass bead, and each first reaction pipeline 110 and each second reaction pipeline 210 can be connected with a gas source equipment, filling eq...

Embodiment 2

[0059] see figure 2 As shown, this embodiment provides a gene sequence sorting and synthesis device 10, which includes a first reactor 100, a second reactor 200, a first sorter 310, a second sorter 320, a second A sorting pipeline 410 and a second sorting pipeline 420 .

[0060] The first reactor 100 includes four first reaction pipelines 110 arranged in parallel, marked as A-1, C-1, T-1 and G-1. The first reaction pipeline 110 has a first reaction channel with a spiral channel structure, and the size of the first reaction channel is larger than the size of the glass beads and smaller than twice the size of the glass beads.

[0061] The second reactor 200 includes four second reaction tubes 210 arranged in parallel, labeled A-2, C-2, T-2 and G-2. The second reaction pipeline 210 has a second reaction channel in a spiral pipeline structure, the size of the second reaction channel is greater than the size of the glass beads and less than twice the size of the glass beads, eac...

Embodiment 3

[0071] This embodiment provides a gene sequence sorting and synthesizing method using the gene sequence sorting and synthesizing device 10 of Example 1. The gene sequence sorting and synthesizing method includes the following steps:

[0072](1) Place several glass beads in several first reaction pipes 110 of the first reactor, add reaction solution respectively in each of the first reaction pipes, the reaction liquid in each of the first reaction pipes 110 The phosphoramidite monomer in the phosphoamidite forms a 3'-5' phosphodiester bond through a condensation reaction, and is connected to a glass bead to form a coupled glass bead, see figure 2 shown.

[0073] (2) Inject synthetic reagents into each first reaction pipeline 110 through liquid injection equipment, and carry out gene synthesis in each first reaction pipeline 110; see image 3 As shown, after the synthesis is completed, the coupled glass beads in each of the first reaction pipelines 110 are sorted by the first ...

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Abstract

The invention discloses a gene sequence sorting synthesizing device and a method thereof. The gene sequence sorting synthesizing device comprises a first reactor, a second reactor, a first sorter, a second sorter, a first sorting pipeline and a second sorting pipeline; the first reactor comprises a plurality of first reaction pipelines which are arranged in parallel; each first reaction pipeline is provided with a first reaction channel; the second reactor comprises a plurality of second reaction pipelines which are arranged in parallel; each second reaction pipeline is provided with a secondreaction channel; each first reaction pipeline and each second reaction pipeline can be communicated with gas source equipment, liquid injection equipment and liquid discharge equipment; each first reaction pipeline is communicated with the first sorter, the first sorter is communicated with the plurality of second reaction pipelines one by one through a plurality of first sorting pipelines, eachsecond reaction pipeline is communicated with the second sorter, and the second sorter is communicated with the plurality of first reaction pipelines one by one through a plurality of second sorting pipelines. The device is high in flux, capable of synthesizing multiple DNA sequences at a time, and easy and convenient to operate.

Description

technical field [0001] The invention relates to the biological field, in particular to a gene sequence sorting and synthesizing device and a sorting and synthesizing method. Background technique [0002] At present, the main manufacturers of DNA synthesizers are all committed to the improvement of machine performance, the development of application fields, and the development and research of high-efficiency and high-yield instruments. [0003] DNA synthesizers will play a key role in changing the structure of proteins and polypeptides, preparing new drugs and opening up new fields related to human diseases and genetic regulation. DNA synthesizers developed according to different chemical methods will also continue to emerge. Because the base pair quantity of most nucleic acids that people need far exceeds the base pair quantity of the longest nucleic acid chain that current DNA synthesizer can synthesize, the DNA synthesizer in the prior art mainly has the following problems...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N15/10
CPCC12N15/1006C12Q2565/631
Inventor 罗杵添
Owner GUANGDONG SHUNDE IND DESIGN INST GUANGDONG SHUNDE INNOVATIVE DESIGN INST
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