Method for driving Methanosarcina to produce methane

Abstract

The invention provides a method for driving Methanosarcina to produce methane, which is characterized by comprising the following steps: inoculating Rhodopseudomonas bacteria and Methanosarcina bacteria into a syntrophic culture medium, adding an inorganic electron donor and CO2, and culturing under an illumination condition. According to the method, light energy and inorganic compounds in the nature can be used as driving force to excite the oxygen-free photosynthetic bacteria to oxidize and produce electrons, then the Methanosarcina bacteria utilize the electrons to realize selective CO2 reduction to produce methane, and the process cost is remarkably reduced.

Description

technical field

[0001] The invention relates to a method for driving methanosarcina to produce methan, belonging to the technical field of microorganisms. Background technique

[0002] The frequent daily activities of human beings and the massive development of industry have gradually exhausted fossil energy and released a large amount of CO 2 . CO 2 A large amount of accumulation will lead to a series of problems such as global warming, sea level rise, and destruction of ecological balance, which seriously threatens the survival and development of human beings. Find an efficient way to convert CO 2 Fixing methane as a valuable energy fuel is an important strategy to protect the ecological environment and alleviate energy shortage.

[0003] Fixed CO 2 The traditional way of producing methane is chemical catalysis. But CO 2 The chemical properties are stable and the reduction path is complex, so it is necessary to find an efficient catalyst to catalyze it into methane....

Examples

Embodiment 1

[0025] (1) Inoculate Methanosarcina barkeri and Rhodopseudomonas palustris with 20% (v / v) and 2% (v / v) respectively into acetic acid medium with pH 7.0, and culture at 37°C; China General Microbiological Culture Collection Center (China General Microbiological Culture Collection Center), the preservation number is CGMCC-1.2180, Methanosarcina barkeri was purchased from the German Microbiological Culture Collection Center (Deutsche Sammlung von Mikroorganismen und Zellkulturen), the preservation number is DSM-800.

[0026] (2) When Methanosarcina barkeri and Rhodopseudomonas palustris grow to late logarithmic phase in step 1, centrifuge at 7500r / min for 10min to collect the bacterial solution, wash the bacteria with normal saline for 3 times, and finally dilute the OD of the bacterial solution with normal saline 600 value to 0.3.

[0027] (3) Take 2% (v / v) Methanosarcina barkeri and Rhodopseudomonas palustris bacterium solution in step 2 and inoculate it into the interoperabili...

Embodiment 2

[0039] To analyze the inorganic photosynthesis of Rhodopseudomonas palustris the fixation of CO in Methanosarcinabarkeri 2 In order to reduce the effect of methanogenesis, a control group experiment was also carried out, including: Methanosarcina barkeri light group, that is, without adding Rhodopseudomonas palustris bacteria, and other operations are the same as in Example 1; Rhodopseudomonas palustris light group, that is, without adding Methanosarcina barkeri bacteria, other operations All the same as in Example 1; Methanosarcinabarkeri-Rhodopseudomonas palustris dark group, that is, cultivated under no light conditions, and other operations are all the same as in Example 1. The test results showed that there was no obvious methane production in the above three control groups.

technical field

[0001] The invention relates to a method for driving methanosarcina to produce methan, belonging to the technical field of microorganisms. Background technique

[0002] The frequent daily activities of human beings and the massive development of industry have gradually exhausted fossil energy and released a large amount of CO 2 . CO 2 A large amount of accumulation will lead to a series of problems such as global warming, sea level rise, and destruction of ecological balance, which seriously threatens the survival and development of human beings. Find an efficient way to convert CO 2 Fixing methane as a valuable energy fuel is an important strategy to protect the ecological environment and alleviate energy shortage.

[0003] Fixed CO 2 The traditional way of producing methane is chemical catalysis. But CO 2 The chemical properties are stable and the reduction path is complex, so it is necessary to find an efficient catalyst to catalyze it into methane....

Examples

Embodiment 1

[0025] (1) Inoculate Methanosarcina barkeri and Rhodopseudomonas palustris with 20% (v / v) and 2% (v / v) respectively into acetic acid medium with pH 7.0, and culture at 37°C; China General Microbiological Culture Collection Center (China General Microbiological Culture Collection Center), the preservation number is CGMCC-1.2180, Methanosarcina barkeri was purchased from the German Microbiological Culture Collection Center (Deutsche Sammlung von Mikroorganismen und Zellkulturen), the preservation number is DSM-800.

[0026] (2) When Methanosarcina barkeri and Rhodopseudomonas palustris grow to late logarithmic phase in step 1, centrifuge at 7500r / min for 10min to collect the bacterial solution, wash the bacteria with normal saline for 3 times, and finally dilute the OD of the bacterial solution with normal saline 600 value to 0.3.

[0027] (3) Take 2% (v / v) Methanosarcina barkeri and Rhodopseudomonas palustris bacterium solution in step 2 and inoculate it into the interoperabili...

Embodiment 2

[0039] To analyze the inorganic photosynthesis of Rhodopseudomonas palustris the fixation of CO in Methanosarcinabarkeri 2 In order to reduce the effect of methanogenesis, a control group experiment was also carried out, including: Methanosarcina barkeri light group, that is, without adding Rhodopseudomonas palustris bacteria, and other operations are the same as in Example 1; Rhodopseudomonas palustris light group, that is, without adding Methanosarcina barkeri bacteria, other operations All the same as in Example 1; Methanosarcinabarkeri-Rhodopseudomonas palustris dark group, that is, cultivated under no light conditions, and other operations are all the same as in Example 1. The test results showed that there was no obvious methane production in the above three control groups.

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