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Selenium-rich polyunsaturated fatty acid-rich algae, product and preparation method

An unsaturated fatty acid and algae technology, applied in biochemical equipment and methods, single-cell algae, microorganism-based methods, etc., can solve the problems of selenium algae toxicity, biomass reduction, and algae enrichment limitation, etc. Side effects, safe absorption and utilization, the effect of eliminating gastrointestinal irritation

Active Publication Date: 2020-04-24
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing production method of selenium-enriched algae is to add selenium at the beginning of fermentation, but selenium usually has certain toxicity to algae, and directly adding selenium at the beginning of fermentation will seriously reduce the biomass and affect the yield of oil , while limiting the amount of selenium-enriched algae

Method used

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  • Selenium-rich polyunsaturated fatty acid-rich algae, product and preparation method
  • Selenium-rich polyunsaturated fatty acid-rich algae, product and preparation method
  • Selenium-rich polyunsaturated fatty acid-rich algae, product and preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The preparation method of the selenium-enriched polyunsaturated fatty acid algae product provided by the present embodiment is as follows:

[0056] Strains: Schizochytrium sp. The preservation number is CCTCC NO: M2015716, and it was preserved in the China Center for Type Culture Collection on November 30, 2015. The preservation address is: Wuhan University Campus, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province, Collection Center of Wuhan University.

[0057] 1 seed activation culture:

[0058] The Schizochytrium mutant strain CCTCC NO:M2015716 was inoculated into the activation medium for cultivation, the cultivation temperature was 25-28°C, the shaking speed of the shaker was 200r / min, and the cultivation time was 48h, so as to obtain the primary seed activation culture solution.

[0059] Used activation medium comprises: glucose 10g / L, sodium glutamate 20g / L, yeast extract 10g / L, sodium chloride 15g / L, magnesium sulfate 0.5g / L, pH natural.

[0060]...

Embodiment 2-18

[0089] The preparation method of embodiment 2-16 is basically the same as embodiment 1, and the main difference of each embodiment is shown in table 1. The difference of embodiment 17,18 and embodiment 1 is that what use is commercially available Schizochytrium, numbering is ATCC20888.

[0090]

[0091]

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Abstract

The invention discloses selenium-rich polyunsaturated fatty acid-rich algae, a product and a preparation method. The preparation method comprises a fermentation step: adding a selenium salt into a fermentation culture medium inoculated with algae. According to the preparation method, a fermentation process is optimized, so that the content of selenium in algae can be effectively increased, and theselenium-rich polyunsaturated fatty acid-rich algae can be prepared.

Description

technical field [0001] The invention relates to the technical field of selenium-enriched fermentation, in particular to a selenium-enriched polyunsaturated fatty acid algae, a product and a preparation method. Background technique [0002] The benefits of selenium to the human body: Selenium (Se) is determined by the World Health Organization and the Chinese Medical Association as the third largest micronutrient and health element that must be supplemented after iodine and zinc in the 21st century. It has anti-oxidation, anti-cancer, detoxification, and Improve immunity and so on. At present, most of the selenium-enriched algae on the market are selenium-enriched spirulina and chlorella, because these two algae have high tolerance to selenium, and selenium-enriched products of other algae are rarely seen. [0003] Polyunsaturated fatty acids (Polyunsaturated fatty acids, PUFA) refers to straight-chain fatty acids containing two or more double bonds and a carbon chain length...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/12C12N1/38Y02A40/818
Inventor 李翔宇陆姝欢熊淑婷余超汪志明
Owner CABIO BIOTECH WUHAN CO LTD