Dual-target site inverse-transcription fluorescent PCR primers, probes and kits for detection of 2019 new coronavirus

A coronavirus, primer-probe technology, applied in biochemical equipment and methods, microbial determination/inspection, resistance to vector-borne diseases, etc., can solve problems such as false negative test results, missed detection, etc.
CN111074005APending Publication Date: 2020-04-28西安博睿康宁生物医学中心有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
西安博睿康宁生物医学中心有限公司
Publication Date
2020-04-28

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Abstract

The invention provides dual-target site inverse-transcription fluorescent PCR primers, probes and kits for detection of 2019 new coronavirus (2019-nCoV). Nucleotide sequence alignment is performed onthe whole genome of the 2019-nCoV, 3 specific nucleotide sequences W1, W2 and W3 are found in N genes of the virus, and are shown in SEQ ID No. 1-3, multiple pairs of primer probes are designed separately for three target sites, and it is found that when a primer probe combination which is designed for the target sites of W1 and W2 and has sequences shown in SEQ ID No. 4-6 and SEQ ID No. 7-9 is applied to double-target site inverse-transcription fluorescent PCR, 2019-nCoV can be detected specifically and sensitively with detection sensitivity of within 10 copies; and no non-specific amplification of 22 clinically-common respiratory pathogens are caused, false-negative results caused by single target spot mutation can be avoided effectively, and good detection ability of specimens is achieved.
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Description

technical field

[0001] The present invention relates to the technical field of virus detection, in particular to target sites for detecting 2019 novel coronavirus, specific primers and probes for the target sites, and the present invention also relates to the use of the primers and probes to detect 2019 novel coronavirus Detection methods and kits. Background technique

[0002] Novel coronavirus (2019-nCoV) is the latest human-pathogenic coronavirus detected in January 2020, and it is the respiratory pathogen that causes pneumonia outbreaks of unknown cause. The clinical manifestations are fever, fatigue and other systemic symptoms, accompanied by dry cough, and dyspnea is common in hospitalized patients; the vital signs of most patients are generally stable when they are admitted to the hospital. Although the virus has not been found to be contagious between humans, it still requires close monitoring to prevent larger infections. From January 11th to 12th, 2020, six sets ...

Claims

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