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Application of deoxyelephantolide or isodeoxyelephantolide in preparation of a medicine used for resisting lung fibrosis

A technology for pulmonary fibrosis and dandelion, applied in the field of medicine, can solve the problems such as no reports and applications of anti-fibrosis treatment yet.

Active Publication Date: 2020-05-01
仁合熙德隆药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Deoxydicholide and isodeoxydicholide are two kinds of natural products, both of which are isomers. There have been many research reports on their activities, but they have not been seen in anti-fibrosis. Reports and Applications in Therapeutics

Method used

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  • Application of deoxyelephantolide or isodeoxyelephantolide in preparation of a medicine used for resisting lung fibrosis
  • Application of deoxyelephantolide or isodeoxyelephantolide in preparation of a medicine used for resisting lung fibrosis

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Experimental program
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Effect test

Embodiment 1

[0016] 1. Test materials

[0017] Deoxydicholide (CAS: 29307-03-7) with a purity of 97.5%, isodeoxydicholide (CAS: 38927-54-7) with a purity of 98.0%, purchased from Shanghai Shushi Biotechnology Co., Ltd. .

[0018] Human embryonic lung fibroblasts HFL1 were purchased from ATCC and used after recovery according to conventional methods.

[0019] Fetal bovine serum and F12K medium were purchased from Gbico, USA.

[0020] Penicillin, streptomycin, and trypsin were purchased from Biyuntian Biotech.

[0021] The brand of 96-well plate and 6-well plate is Corning.

[0022] 2. Test method

[0023] 1. Culture of human embryonic lung fibroblasts

[0024] Place human embryonic lung fibroblasts HFL1 in F12K medium containing 10% fetal bovine serum, 100 U / mL penicillin and 100 μg / mL streptomycin at 37°C, 5% CO 2 Routine culture in the incubator, with 0.25% trypsin digestion passage. Human embryonic lung fibroblasts HFL1 in the logarithmic growth phase were used for subsequent expe...

Embodiment 2

[0040] Formula composition: an anti-pulmonary fibrosis tablet, the formula includes 10 mg of deoxycholate or isodeoxycholine, 90 g of starch, and 3 g of magnesium stearate.

[0041] Preparation process: take deoxydicholide or isodeoxydicholide, add starch and magnesium stearate, mix evenly, make granules, dry, and compress into tablets.

Embodiment 3

[0043] Formula composition: an anti-pulmonary fibrosis capsule, the formula includes 10 mg of deoxycholate or isodeoxycholine, 90 g of starch, and 3 g of magnesium stearate.

[0044] Preparation process: take deoxydicholide or isodeoxydicholide, add starch and magnesium stearate, mix evenly, make granules, dry, and pack into capsules.

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Abstract

The invention discloses an application of deoxyelephantolide or isodeoxyelephantolide in preparation of a medicine used for resisting lung fibrosis. Researches find that the deoxyelephantolide or theisodeoxyelephantolide can be used for effectively inhibiting the proliferation of human embryonic lung fibroblasts HFL1. Western Blot results show that the proliferation inhibition effect of the deoxyelephantolide or the isodeoxyelephantolide on human embryonic lung fibroblasts HFL1 is likely to be related with the situation that the human embryonic lung fibroblasts is blocked in a G1 phase and can not realize cell division and proliferation through down regulation of the expression of cyclin D1 and CDK4. Therefore, the deoxyelephantolide or the isodeoxyelephantolide has the prospect of beingdeveloped into the medicine used for resisting the lung fibrosis.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the discovery of anti-pulmonary fibrosis drugs, in particular to the application of deoxydicholactone or isodeoxydichocholide in the preparation of anti-pulmonary fibrosis drugs. Background technique [0002] Pulmonary fibrosis is an immune-mediated chronic inflammatory disease characterized by inflammatory cell infiltration in the alveolar interstitium, abnormal proliferation of fibroblasts, and collagen deposition, and is a common outcome of many pulmonary interstitial diseases with different etiologies. , the clinical manifestations are mainly progressive dyspnea, and the patient eventually died of respiratory failure. At present, there is still a lack of effective treatment methods. Therefore, finding effective drugs against pulmonary fibrosis has become an urgent need for current medicine. [0003] Pulmonary fibroblasts are differentiated from embryonic mesenchymal cells and constitute...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P11/00
CPCA61K31/365A61P11/00
Inventor 朱阳晨赵玲
Owner 仁合熙德隆药业有限公司
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