Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture medium applied to in-vitro culture of balantidium ctenopharyngodoni, preparation method of culture medium and in-vitro culture method of balantidium ctenopharyngodoni

A technology of bagworm and medium for grass carp, which is applied in general culture methods, biochemical equipment and methods, microorganisms, etc., can solve the problems that there are no bags of grass carp, achieve strong vitality and fecundity, less equipment, Achieve the effect of division and proliferation

Active Publication Date: 2018-10-12
INST OF AQUATIC LIFE ACAD SINICA
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the present invention was made, the reports on bagworms at home and abroad mainly focused on morphology and epidemiology research, and there were no research reports on the culture medium components and culture methods of bagworms cultured in vitro at present.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture medium applied to in-vitro culture of balantidium ctenopharyngodoni, preparation method of culture medium and in-vitro culture method of balantidium ctenopharyngodoni
  • Culture medium applied to in-vitro culture of balantidium ctenopharyngodoni, preparation method of culture medium and in-vitro culture method of balantidium ctenopharyngodoni
  • Culture medium applied to in-vitro culture of balantidium ctenopharyngodoni, preparation method of culture medium and in-vitro culture method of balantidium ctenopharyngodoni

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A kind of culture medium that is applied to the culture medium of bagworm of grass carp intestines in vitro of the present embodiment, the formula composition and content of described culture medium are as follows: Ren's solution 100ml, yeast extract 0.5g, Peptone 1.0g, fetal bovine serum 3.5ml, horse serum 10ml, Bacillus licheniformis bacterial liquid 335μl, sterile starch 300mg;

[0041] Wherein: the formula composition and content of described Ren's liquid are as follows: sodium chloride (NaCl) 6.5g, potassium chloride (KCl) 0.14g, calcium chloride (CaCl) 2 ) 0.12g, sodium bicarbonate (NaHCO 3 ) 0.2g, sodium dihydrogen phosphate (NaH 2 PO 4 ) 0.01g, distilled water 1000ml.

[0042]The above-mentioned Ren's solution is prepared by the following method, comprising the steps of:

[0043] (i) 6.5 g of sodium chloride, 0.14 g of potassium chloride, 0.2 g of sodium bicarbonate, and 0.01 g of sodium dihydrogen phosphate were dissolved in 999 ml of distilled water in tu...

Embodiment 2

[0061] A kind of culture medium that is applied to the culture medium of bagworm of grass carp intestines in vitro of the present embodiment, the formula composition and content of described culture medium are as follows: Ren's solution 100ml, yeast extract 0.5g, Peptone 1.0g, fetal bovine serum 3ml, horse serum 6ml, Bacillus licheniformis bacteria liquid 300μl, sterile starch 200mg;

[0062] Wherein: the formula and the preparation method of described Ren's liquid are identical with embodiment 1.

[0063] The preparation method of the medium applied to the in vitro culture of bagworms of grass carp as described above in this embodiment comprises the following steps:

[0064] (a) Accurately weigh 0.5 g of yeast extract and Put 1.0g of peptone in a 250ml Erlenmeyer flask, add 100ml of Ren's solution, adjust the pH to 7.0-7.5, seal it with foil paper, sterilize at 121°C for 20min at high temperature and high pressure, and store the culture medium at 4°C after cooling;

[006...

Embodiment 3

[0078] A kind of culture medium that is applied to the culture medium of bagworm of grass carp intestines in vitro of the present embodiment, the formula composition and content of described culture medium are as follows: Ren's solution 100ml, yeast extract 0.5g, Peptone 1.0g, fetal bovine serum 6ml, horse serum 9ml, Bacillus licheniformis bacterial liquid 335μl, sterile starch 250mg;

[0079] Wherein: the formula and the preparation method of described Ren's liquid are identical with embodiment 1.

[0080] The preparation method of the medium applied to the in vitro culture of bagworms of grass carp as described above in this embodiment comprises the following steps:

[0081] (a) Accurately weigh 0.5 g of yeast extract and Put 1.0g of peptone in a 250ml Erlenmeyer flask, add 100ml of Ren's solution, adjust the pH to 7.0-7.5, seal it with foil paper, sterilize at 121°C for 20min at high temperature and high pressure, and store the culture medium at 4°C after cooling;

[00...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a culture medium applied to in-vitro culture of balantidium ctenopharyngodoni, a preparation method of the culture medium and a culture method of the balantidium ctenopharyngodoni and belongs to the technical field of in-vitro culture of parasitic protozoa. The culture medium disclosed by the invention is prepared from 100ml of Ringer's solution, 0.5g of yeast extract, 1.0g of pulse peptone, 3ml to 6ml of fetal calf serum, 6ml to 10ml of horse serum, 300 to 500 microliters of bacillus licheniformis bacterial solution and 200mg to 300mg of sterile starch. The culture method comprises the following polypide culture steps: inoculating the prepared culture medium with extracted balantidium ctenopharyngodoni, charging the culture medium with nitrogen gas, then, carryingout sealing, carrying out culture for 48 to 72 hours at the temperature of 15 DEG C, then, transferring polypides to another fresh culture medium, charging the culture medium with nitrogen gas, then,carrying out sealing, carrying out culture at the temperature of 15 DEG C, and repeating in cycles so as to continuously propagate the polypides. According to the culture medium disclosed by the invention, the preparation method is simple, the stability is good, the division and propagation of the polypides in the culture medium can be achieved, and thus, a foundation is laid for the physiological and experimental ecological researches of the balantidium ctenopharyngodoni.

Description

technical field [0001] The invention belongs to the technical field of in vitro culture of parasitic protozoa, in particular, the invention relates to a medium for in vitro culture of bagworms, a preparation method thereof and an in vitro culture method of bagworms. Background technique [0002] Grass carp intestinal bagworm is an obligate parasitic ciliate in the grass carp intestine first discovered and named by Mr. Chen Qiliu (1955). Molnár and Reinhardt (1978) used histological methods to observe the intestinal tract of grass carp infected with bagworms and found that it parasitizes in the mucosal folds and contents of the hindgut, and the large number of worms will lead to catarrh in the hindgut of grass carp. enteritis and may inflame other bowel segments. Li Ming et al. (2007, 2014) observed the morphology and ultrastructure of bagworms by hematoxylin staining, scanning electron microscopy and transmission electron microscopy, and described the morphological characte...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033C12N5/0068C12N1/10C12N1/00
Inventor 王桂堂赵威山李明吴山功邹红李文祥熊凡
Owner INST OF AQUATIC LIFE ACAD SINICA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com