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Thymus-dependent lymphocyte antigen epitope peptides of hepatitis B virus antigens and application thereof

A hepatitis B virus and antigen epitope technology, applied in the fields of medical immunology and infectious diseases, can solve the problems of restricting personalized detection and restricting the research of HBV-specific T cell action

Active Publication Date: 2020-05-08
南京大户生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]However, there are still very few HBV antigen peptides that are clearly presented by various HLA molecules and can stimulate the body to cause T cell responses, thus limiting the research on The detection of specific T cells in hepatitis B patients carrying different HLA alleles also limits the research on the role of HBV-specific T cells in the development of hepatitis B, and limits the HBV antigen peptides based on individual differences in HLA genes and their presentation. Differentiated personalized detection and precise immunotherapy

Method used

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  • Thymus-dependent lymphocyte antigen epitope peptides of hepatitis B virus antigens and application thereof
  • Thymus-dependent lymphocyte antigen epitope peptides of hepatitis B virus antigens and application thereof
  • Thymus-dependent lymphocyte antigen epitope peptides of hepatitis B virus antigens and application thereof

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Embodiment 1

[0045] HLA-A0201, A1101, A2402, A3101, A0206, A0207, A3303, A3001, A0203, A1102, A0301, A0101, A2601 molecule-restricted HBV-specific antigen peptides of the present invention, the sequence is screened and identified through the following steps:

[0046] 1. Online virtual prediction of dominant T cell epitope peptides of 4 HBV antigens restricted by two HLA-A molecules

[0047] Four HBV proteins were selected: surface antigen (HBsAg), e antigen (HBeAg, including the sequence of HBcAg), DNA polymerase (HBpol) and X protein (HBx), and their amino acid sequences were obtained by searching the UniProt global protein resource database. HBV protein sequence of the most infected HBV type C; then through six commonly used epitope peptide prediction databases including SYFPEITHI, BIMAS, SVMHC, IEDB, NETMHC, EPIJEN, etc. A3001, A0203, A1102, A0301, A0101, A2601 molecularly restricted T cell epitope peptides for each of the above HBV proteins for virtual prediction, in which the SVMHC da...

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Abstract

The invention belongs to the fields of medical immunology and infectious disease science, and particularly relates to thymus-dependent lymphocyte antigen epitope peptides of 189 hepatitis B virus antigens and application thereof. The antigen epitope peptides are characterized in that antigen peptides respectively restricted by molecules of HLA-A0201, HLA-A1101, HLA-A2402, HLA-A0207, HLA-A3101, HLA-A0206, HLA-A3303, HLA-A3001, HLA-A0203, HLA-A1102, HLA-A0301, HLA-A2601 and HLA-A0101 can be specifically bonded with cytotoxic thymus-dependent lymphocytes to stimulate the activation, proliferationand differentiation of the cytotoxic thymus-dependent lymphocytes so as to achieve the immunological effect action of resisting hepatitis B viruses; and the antigen peptides can be used for preparingtherapeutic and preventative vaccines for hepatitis B virus infection, can also be used for preparing detection reagents for detecting hepatitis B virus specific cytotoxic thymus-dependent lymphocytes, and have potential application values to prevention, treatment and diagnosis of hepatitis B.

Description

technical field [0001] The invention belongs to the fields of medical immunology and infectious diseases, and particularly relates to thymus-dependent lymphocyte antigen epitope peptides of 189 hepatitis B virus antigens and applications thereof. Background technique [0002] Hepatitis B virus (HBV) infection is one of the most common infectious diseases, which seriously endangers human health. According to statistics from the World Health Organization in July 2015, it is estimated that 1 / 3 of the world's population has been infected with HBV, and about 248 million HBV carriers may develop liver cirrhosis and primary hepatocellular carcinoma in the later stage. About 780,000 HBV-infected patients die each year due to disease progression to cirrhosis, liver failure, or liver cancer. [0003] The HBV genome is composed of incomplete double-stranded circular DNA, the long negative strand contains 3020-3320 bases, and the short positive strand contains 1700-2800 bases. It incl...

Claims

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Application Information

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IPC IPC(8): C07K14/02A61K39/29A61P1/16A61P31/20G01N33/576G01N33/569
CPCC07K14/005A61K39/12A61P1/16A61P31/20G01N33/5764G01N33/5762G01N33/56966C12N2730/10122C12N2730/10134A61K2039/53Y02A50/30A61K2039/572
Inventor 沈传来周子宁丁艳
Owner 南京大户生物科技有限公司