New three-dimensional test method for common-optical-path F-P cavity phase enhanced cell absorptivity

An F-P, absorptivity technology, applied in image detector methods and image signal processing, measurement devices, material analysis by optical means, etc. question

Pending Publication Date: 2020-05-08
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

[0011] Patent CN201710518263.5 discloses a three-dimensional refractive index tomography microscopic imaging system and its method, which can restore the three-dimensional refractive index information of the sample, but in the imaging optical path, it is essentially different from the present invention, and the required devices are more It is complex and difficult to obtain the three-...

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  • New three-dimensional test method for common-optical-path F-P cavity phase enhanced cell absorptivity
  • New three-dimensional test method for common-optical-path F-P cavity phase enhanced cell absorptivity
  • New three-dimensional test method for common-optical-path F-P cavity phase enhanced cell absorptivity

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Embodiment 1

[0061] Figure 2b An embodiment of the digital holographic recording optical path of a common optical path F-P cavity is given. The optical path includes a light source 21 , an attenuator 22 , a beam expander 23 , an F-P etalon 24 , a cell to be measured 25 , a microscope objective lens 26 , a CCD 27 and a computer 28 . The light beam starts from the light source 21, passes through the attenuator 22, the energy of the light beam is weakened, and the light beam passes through the beam expander 23, and the diameter of the light beam is expanded. After passing through the cell 25 to be tested multiple times, each beam of transmitted light passing through the F-P etalon 24 passes through the microscope objective 26, and then interferes and superimposes on the CCD 27. The digital hologram of the interference is recorded by the CCD 27 and stored in the computer 28.

[0062] In this embodiment, preferably, the light source 21 adopts a laser light source with a wavelength of 532 nm, ...

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Abstract

The invention provides a new three-dimensional test method for a common-optical-path F-P cavity enhanced cell absorptivity. The method is characterized by comprising digital hologram recording based on an F-P cavity, numerical reconstruction, error processing and three-dimensional absorptivity distribution reconstruction. The invention mainly provides a new three-dimensional test method for the common-optical-path F-P cavity enhanced cell absorptivity, and compared with a traditional microscopic imaging method, the new three-dimensional test method has higher sensitivity. The method provided by the invention has the advantages of simple structure, high sensitivity and precise measurement. The method can be used for high-resolution three-dimensional microscopic imaging of biological cells,and can be widely applied to nondestructive, label-free and non-contact three-dimensional tomography and the like of optical transparent objects.

Description

[0001] (1) Technical field [0002] The invention relates to a new method for three-dimensional testing of the common optical path F-P cavity phase-enhanced cell absorptivity, which can be used for high-resolution three-dimensional microscopic imaging of biological cells, and can be widely used in non-destructive, label-free and non-contact optically transparent objects. The three-dimensional tomographic imaging of the formula and the like belong to the technical field of microscopic imaging. [0003] (2) Background technology [0004] The three-dimensional absorptivity distribution of the cells to be tested is an important inherent attribute. For optically transparent cells to be tested, the three-dimensional absorptivity distribution can reflect the microstructure, density and other information of the sample. Labeled, non-contact 3D tomography. [0005] In contemporary life science research, fluorescence imaging is usually used to label the samples to be tested. However, in...

Claims

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Application Information

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IPC IPC(8): G01N21/17G03H1/00G03H1/04G03H1/22
CPCG01N21/17G03H1/0005G03H1/0443G03H1/22G01N2021/177G01N2021/1787G03H2001/0038G03H2001/005
Inventor 苑立波孟令知李晟
Owner GUILIN UNIV OF ELECTRONIC TECH
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