Equipment and method for testing lignocellulose components of edible fungus culture medium
A technology of lignocellulose and cultivation substrate, which is applied in the field of testing equipment for lignocellulose components of edible fungus cultivation substrate, can solve the problems of large hydrolysis reaction system, hidden danger of sulfuric acid overflow, unfavorable sample determination, etc., to improve hydrolysis rate and avoid temperature Effect
Pending Publication Date: 2020-05-08
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology
[0004] However, in the process of determining the lignocellulose of Agaricus bisporus cultivation substrate with the method established by NREL, it was found that the hydrolysis reaction of this method needs to be carried out in a pressure vessel, and there is a hidden danger of sulfuric acid overflowing during the high-temperature and high-pressure hydrolysis process, and the hydrolysis The reaction system is relatively large, which is not conducive to the determination of large batches of samples
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[0060] The following description serves to disclose the invention to enable those skilled in the art to practice the invention. The preferred embodiments described below are given by way of example only, and other obvious modifications will occur to those skilled in the art. The basic principles of the invention defined in the following description may be applied to other embodiments, variations, improvements, equivalents, and other technical solutions without departing from the spirit and scope of the invention.
[0061] It should be understood by those skilled in the art that in the disclosure of the present invention, the terms "portrait", "horizontal", "upper", "lower", "front", "rear", "left", "right", " The orientation or positional relationship indicated by vertical, horizontal, top, bottom, inner, outer, etc. is based on the orientation or positional relationship shown in the accompanying drawings, which are only for the convenience of describing the present invention ...
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The invention provides equipment for testing a lignocellulose component of an edible fungus culture medium. The equipment comprises a reaction device for testing the lignocellulose component of the edible fungus culture medium, a high performance liquid anion chromatograph and a lignin reaction device. According to the reaction device for testing the lignocellulose component of the edible fungus culture medium, an edible fungus culture medium sample is hydrolyzed in 10mL of 15.4% (w / w) sulfuric acid solution at a temperature of 100 DEG C to form a second supernatant and a second residue. And the high performance liquid anion chromatograph is used for testing the monosaccharide content of the second supernatant and calculating cellulose and hemicellulose. And the lignin reaction device is used for testing the second residue and calculating the acid-insoluble lignin.
Description
technical field [0001] The invention relates to the field of biotechnology of a cultivation substrate, in particular to a testing device and a method for lignocellulose components of an edible fungus cultivation substrate. Background technique [0002] The earth is rich in lignocellulose renewable resources. The amount of dry matter produced by plants in the world is as high as 1.55 × 10 per year 11 tons, of which the total amount of cellulose and hemicellulose is about 8.5×10 10 Ton. The degradation and transformation of lignocellulose by fungi is an effective way to solve the energy shortage crisis. Edible fungi have a strong ability to degrade lignocellulose, and at the same time solve the problems of food shortage, energy crisis and environmental pollution. In recent years, in the process of comparing the effects of different strains, different raw materials, and different cultivation methods on the degradation efficiency of lignocellulose from the aspects of lignoce...
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IPC IPC(8): G01N30/02G01N30/36G01N30/86G01N5/04
CPCG01N30/02G01N30/36G01N30/8675G01N5/04
Inventor 王倩黄建春隽加香肖婷婷陈明杰张津京陈辉宋晓霞
Owner SHANGHAI ACAD OF AGRI SCI