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Method for extracting high-purity stevioside by adopting simulated moving bed chromatography

A technology of simulating moving bed and steviol glycosides, which is applied in the field of purification of natural plant effective components, can solve the problems of large amount of resin and mobile phase, long resin regeneration cycle, and reduced product yield, so as to achieve less loss of total glycosides and shorten The effect of increasing the production cycle and increasing the settling velocity

Inactive Publication Date: 2020-05-15
内蒙古昶辉生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In these processes, the water consumption is large during water soaking and extraction, which requires distilled water concentration and high energy consumption; when the flocculant is used to remove impurities, a large amount of precipitation will be generated after adding flocculant to the leachate, although most impurities can be removed, but it will also be adsorbed. A part of steviol glycosides reduces the product yield and produces a large amount of salt at the same time; column chromatography purification is a key step of the process, in addition to removing a small amount of pigment, tannin and other sugars remaining in the stevioside extract, it can also desalt and other impurities , but the amount of resin and mobile phase is large, the utilization rate of resin is low, and the resin needs to be regenerated. The regeneration cycle of desalination and decolorization resin is generally very long, and a large amount of waste water is generated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for extracting high-purity steviol glycosides using simulated moving bed chromatography, comprising the steps of:

[0033] (1) Extraction of raw materials: Select high-quality dry leaves of Stevia rebaudiana through screening and carry out two-stage countercurrent extraction at a water temperature of 80°C, and the extract enters the next process;

[0034] (2) Flocculation process: place the extract in a water bath at 42°C and stir at a constant temperature for 16 minutes at a stirring speed of 35 rpm, then add a flocculant, adjust the pH to 9, let stand for 35 minutes, and filter to obtain a clarified water extract of Stevia rebaudiana;

[0035] The flocculant, its raw materials include by weight: 6.6 parts of chitosan, 18.5 parts of lignin, 15.8 parts of ammonium persulfate, 23 parts of polyaluminum ferric sulfate, 23.5 parts of aluminum citrate, 4.2 parts of magnesium sulfate, calcium chloride 3.3 parts, 18.2 parts of iron polysilicate, 3.6 parts of sodium carb...

Embodiment 2

[0046] A method for extracting high-purity steviol glycosides using simulated moving bed chromatography, comprising the steps of:

[0047] (1) Raw material extraction: select high-quality dry leaves of Stevia rebaudiana through screening and carry out two-stage countercurrent extraction at a water temperature of 75°C, and the extract enters the next process;

[0048] (2) Flocculation process: place the extract in a water bath at 46°C and stir at a constant temperature for 16 minutes at a stirring speed of 55 rpm, then add a flocculant, adjust the pH to 9, let it stand for 35 minutes, and filter to obtain a clarified water extract of Stevia rebaudiana;

[0049] The flocculant, its raw materials include by weight: 7.3 parts of chitosan, 18.4 parts of lignin, 16.6 parts of ammonium persulfate, 23.5 parts of polyaluminum ferric sulfate, 23 parts of aluminum citrate, 3.5 parts of magnesium sulfate, calcium chloride 2.6 parts, 19.6 parts of iron polysilicate, 3.9 parts of sodium car...

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Abstract

The invention discloses a method for extracting high-purity stevioside by adopting simulated moving bed chromatography. The method for extracting the high-purity stevioside by adopting the simulated moving bed chromatography comprises the following steps: extracting raw materials; carrying out flocculation; carrying out adsorption and separation; carrying out concentration; carrying out crystallization; carrying out centrifugal separation so as to obtain a stevioside concentration; separating stevioside from the stevioside concentration by performing simulated moving bed chromatography so as to obtain extraction liquid and raffinate; separately concentrating the extraction liquid and the raffinate by using film rotary evaporators until ethanol smell is eliminated; and then, washing the concentrates by using ethanol, and drying the washed concentrates in a vacuum drying chamber until the constant weight is reached. The method for extracting the high-purity stevioside by adopting the simulated moving bed chromatography disclosed by the invention is capable of achieving a total glycoside content higher than 99% and a stevioside A content higher than 98%; and thus, significant purification effects are realized.

Description

technical field [0001] The invention relates to a method for purifying effective components of natural plants, in particular to a method for extracting high-purity steviol glycosides by using simulated moving bed chromatography. Background technique [0002] Stevia rebaudiana (stevia rebaudiana), also known as stevia and sugar grass, belongs to Compositae (Comppositae), Stevia genus, and its origin is in subtropical South America. Since 1976, scientific research units such as Nanjing Zhongshan Botanical Garden and Chinese Academy of Agricultural Sciences have successively introduced stevia from Japan for trial planting. Stevia contains 14 kinds of trace elements and 32 kinds of nutrients. It is not metabolized, not accumulated, not carcinogenic, and non-toxic in the body. Its safety has been recognized by international organizations such as FAO and WHO, and the United States. Sugar source, but also a good source of nutrition. [0003] Steviol glycosides, referred to as ste...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/08
CPCC07H15/256C07H1/08
Inventor 张成亮孙立姜鸿奇鞠向慧廖小雪张树元
Owner 内蒙古昶辉生物科技股份有限公司
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