Method for extracting high-purity stevioside by adopting simulated moving bed chromatography

A technology of simulating moving bed and steviol glycosides, which is applied in the field of purification of natural plant effective components, can solve the problems of large amount of resin and mobile phase, long resin regeneration cycle, and reduced product yield, so as to achieve less loss of total glycosides and shorten The effect of increasing the production cycle and increasing the settling velocity

CN111153950AInactive Publication Date: 2020-05-15内蒙古昶辉生物科技股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2020-05-15
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention discloses a method for extracting high-purity stevioside by adopting simulated moving bed chromatography. The method for extracting the high-purity stevioside by adopting the simulated moving bed chromatography comprises the following steps: extracting raw materials; carrying out flocculation; carrying out adsorption and separation; carrying out concentration; carrying out crystallization; carrying out centrifugal separation so as to obtain a stevioside concentration; separating stevioside from the stevioside concentration by performing simulated moving bed chromatography so as to obtain extraction liquid and raffinate; separately concentrating the extraction liquid and the raffinate by using film rotary evaporators until ethanol smell is eliminated; and then, washing the concentrates by using ethanol, and drying the washed concentrates in a vacuum drying chamber until the constant weight is reached. The method for extracting the high-purity stevioside by adopting the simulated moving bed chromatography disclosed by the invention is capable of achieving a total glycoside content higher than 99% and a stevioside A content higher than 98%; and thus, significant purification effects are realized.
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Description

technical field

[0001] The invention relates to a method for purifying effective components of natural plants, in particular to a method for extracting high-purity steviol glycosides by using simulated moving bed chromatography. Background technique

[0002] Stevia rebaudiana (stevia rebaudiana), also known as stevia and sugar grass, belongs to Compositae (Comppositae), Stevia genus, and its origin is in subtropical South America. Since 1976, scientific research units such as Nanjing Zhongshan Botanical Garden and Chinese Academy of Agricultural Sciences have successively introduced stevia from Japan for trial planting. Stevia contains 14 kinds of trace elements and 32 kinds of nutrients. It is not metabolized, not accumulated, not carcinogenic, and non-toxic in the body. Its safety has been recognized by international organizations such as FAO and WHO, and the United States. Sugar source, but also a good source of nutrition.

[0003] Steviol glycosides, referred to as ste...

Examples

Embodiment 1

[0032] A method for extracting high-purity steviol glycosides using simulated moving bed chromatography, comprising the steps of:

[0033] (1) Extraction of raw materials: Select high-quality dry leaves of Stevia rebaudiana through screening and carry out two-stage countercurrent extraction at a water temperature of 80°C, and the extract enters the next process;

[0034] (2) Flocculation process: place the extract in a water bath at 42°C and stir at a constant temperature for 16 minutes at a stirring speed of 35 rpm, then add a flocculant, adjust the pH to 9, let stand for 35 minutes, and filter to obtain a clarified water extract of Stevia rebaudiana;

[0035] The flocculant, its raw materials include by weight: 6.6 parts of chitosan, 18.5 parts of lignin, 15.8 parts of ammonium persulfate, 23 parts of polyaluminum ferric sulfate, 23.5 parts of aluminum citrate, 4.2 parts of magnesium sulfate, calcium chloride 3.3 parts, 18.2 parts of iron polysilicate, 3.6 parts of sodium carb...

Embodiment 2

[0046] A method for extracting high-purity steviol glycosides using simulated moving bed chromatography, comprising the steps of:

[0047] (1) Raw material extraction: select high-quality dry leaves of Stevia rebaudiana through screening and carry out two-stage countercurrent extraction at a water temperature of 75°C, and the extract enters the next process;

[0048] (2) Flocculation process: place the extract in a water bath at 46°C and stir at a constant temperature for 16 minutes at a stirring speed of 55 rpm, then add a flocculant, adjust the pH to 9, let it stand for 35 minutes, and filter to obtain a clarified water extract of Stevia rebaudiana;

[0049] The flocculant, its raw materials include by weight: 7.3 parts of chitosan, 18.4 parts of lignin, 16.6 parts of ammonium persulfate, 23.5 parts of polyaluminum ferric sulfate, 23 parts of aluminum citrate, 3.5 parts of magnesium sulfate, calcium chloride 2.6 parts, 19.6 parts of iron polysilicate, 3.9 parts of sodium car...