Ultrasonic cell collecting method and device
An ultrasonic and cell technology, applied in the direction of microorganism-based methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of long time-consuming cell harvesting and complicated processing technology, and achieve improved virus yield and simple process , the effect of reducing stress
Pending Publication Date: 2020-05-29
罗火生
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Problems solved by technology
[0003] The purpose of the present invention is to overcome the shortcomings of the prior art, seek to design a method and device for harvesting cells based on ultrasonic waves, and solve the problems of long time-consuming and complicated processing techniques in the existing harvesting cell methods
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Embodiment 1
[0024] A method for harvesting cells by ultrasonic waves, which uses ultrasonic waves to peel cells to be harvested from the wall of a cell factory, and the peeled cells are discharged from the liquid outlet (or liquid inlet) of the cell factory together with the culture medium.
[0025] An ultrasonic cell harvesting device includes an ultrasonic device and a cell factory 1. The vibrating end 2 of the ultrasonic device is placed outside the cell factory, and the vibration of the cell factory is realized by generating ultrasonic waves, and then the cells are peeled off from the wall of the cell factory.
[0026] The vibrating end 2 of the ultrasonic device is close to the outer wall of the cell factory, or has a certain distance from the outer wall of the cell factory. In either case, as long as the ultrasonic waves generated by the ultrasonic device can pass through the vibrating cell factory, the cells can be peeled off from the inner wall of the cell factory. . In order to a...
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The invention discloses an ultrasonic cell collecting method. To-be-collected cells are stripped from walls of a cell factory by using ultrasonic waves, and the stripped cells are discharged from thecell factory together with a culture solution. Meanwhile, the invention discloses a corresponding cell collecting device comprising an ultrasonic device and the cell factory, a vibration end of the ultrasonic device is placed on the outer side of the cell factory and realizes vibration of the cell factory by generating ultrasonic waves so as to strip the cells from the walls of the cell factory, and the vibration end is controlled by a horizontal movement device and a vertical movement device to move horizontally and vertically relative to the cell factory in the process to complete strippingof different layers of the cell factory. The process is simple, short in time, high in efficiency, small in virus loss and capable of reducing the contamination risk and increasing the virus yield; and besides, part of cells can be disrupted in the ultrasonic collecting process, and the pressure on the following disruption step is reduced, and the ultrasonic cell collecting method is particularlyapplicable to the batched cell culture tool such as the cell factory.
Description
technical field [0001] The invention relates to the technical field of biological products, in particular to a method for harvesting cells in a cell factory by using ultrasonic technology. Background technique [0002] The cell factory is a commonly used cell culture tool in the biological and pharmaceutical industries. It is suitable for industrial mass production of vaccines, such as hepatitis A vaccine and Japanese encephalitis vaccine. Most of the cells grow on the wall during the culture process. When preparing vaccines, especially for the preparation of some intracellular virus vaccines with strong cell binding, such as rotavirus, EV71 virus, CA16 virus, hepatitis A virus , herpes virus, etc., the cultured cells need to be stripped and collected from culture tools such as cell factories, spinner bottles, or Kirschner flasks, and crushed to obtain free virus particles. At present, there are mainly two methods for collecting cells: one is the freezing method, specifical...
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IPC IPC(8): C12N13/00C12N7/00C12M1/42C12M1/36C12R1/93
CPCC12N13/00C12N7/00C12M23/52C12M33/08C12M41/00C12N2710/16751
Inventor 罗火生马颖罗成周栋罗鸣
Owner 罗火生