IbAITR5 gene derived from ipomoea batatas, protein encoded by IbAITR5 gene and application of protein

A protein and transgenic plant technology, applied in the IbAITR5 gene and the protein encoded by it and its application fields, can solve the problems of difficult stress tolerance traits and the like

Active Publication Date: 2020-06-02
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is relatively difficult to cultivate and improve stress tolerance traits by traditional breeding techniques, and it is difficult to obtain excellent drought-resistant varieties

Method used

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  • IbAITR5 gene derived from ipomoea batatas, protein encoded by IbAITR5 gene and application of protein
  • IbAITR5 gene derived from ipomoea batatas, protein encoded by IbAITR5 gene and application of protein
  • IbAITR5 gene derived from ipomoea batatas, protein encoded by IbAITR5 gene and application of protein

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Embodiment 1, the acquisition of IbAITR5 gene

[0100] 1. Acquisition of cDNA template

[0101] The total RNA of the sweet potato line Xushu 55-2 test-tube seedlings was extracted with a plant total RNA extraction kit, and the first-strand cDNA was reverse-transcribed from the total RNA with a PrimeScriptTM RT reagent Kit with gDNA Eraser kit.

[0102] 2. Using the cDNA obtained in step 1 as a template, design and artificially synthesize primers 3GSP-1 and 3GSP-2, use the RACE method to amplify a 3'-RACE fragment of about 300bp, and combine the 3'-RACE fragment with the cloning vector pMD19- T connection to obtain recombinant plasmid 2. The recombinant plasmid 2 was sequenced to obtain the nucleotide sequence of the 3'-RACE fragment. The primer sequences are as follows:

[0103] 3GSP-1:5'-CGTAAAGCTACCACCGTCCAG-3'

[0104] 3GSP-2:5′-GTGATGTGTGGAGAATGAGCG-3′

[0105] 3. Design and artificially synthesize primers 5GSP-1 and 5GSP-2, use the cDNA obtained in step 1 as a...

Embodiment 2

[0112] Example 2. Application of IbAITR5 protein in improving plant drought resistance.

[0113] 1. Construction of recombinant plasmid pCAMBIA super1300-IbAITR5-GFP

[0114] 1. Artificially synthesized double-stranded DNA molecules shown in positions 1 to 987 from the 5' end of SEQ ID NO.1 in the sequence listing. Using the double-stranded DNA molecule as a template, OE-F-Xba I: 5'-GC TCTAGA ATGGATGGGCGAGGG GG-3' (underlined is the recognition sequence of restriction endonuclease Xba I) and OE-R-Pst I: 5'-AA CTGCAG AAGG ATAGAGCAGCCCTGTA-3' (the underline is the recognition sequence of restriction endonuclease Pst I) is used as a primer for PCR amplification to obtain a double-strand containing restriction endonuclease XbaI at the N-terminus and restriction endonuclease PstI at the C-terminus DNA molecule.

[0115] 2. Digest the vector pCAMBIA super1300-GFP with restriction endonucleases Xba I and Pst I, and recover the vector backbone 1 of about 10783 bp.

[0116] 3. Th...

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Abstract

The invention discloses an IbAITR5 gene derived from ipomoea batatas, a protein encoded by the IbAITR5 gene and application of the protein. The invention firstly discloses the IbAITR5 gene derived from the ipomoea batatas; and the gene is a DNA molecule as shown in SEQ ID NO. 2 or a DNA molecule with a coding sequence as shown in SEQ ID NO. 2. The invention further discloses the protein coded by the above-mentioned gene and the application of the protein. The IbAITR5 protein and the coding gene thereof are discovered; the coding gene of the IbAITR5 protein is introduced into the ipomoea batatas to obtain transgenic ipomoea batatas plants overexpressing IbAITR5; the transgenic plants are subjected to drought stress treatment, and the drought resistance of the transgenic plants is enhanced;and the IbAITR5 protein and the coding gene thereof have important application value in improvement of drought resistance research of plants, and have wide application space and market prospects in the agricultural field.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the IbAITR5 gene derived from sweet potato and its encoded protein and application. Background technique [0002] Plants may be affected by a variety of adversity stresses throughout their growth and development stages, among which abiotic stresses such as drought, salinity, low temperature, and high temperature, and biotic stresses such as pests and diseases significantly affect plant growth and crop yield. The research on plant stress resistance has always been a hot spot in the field of botany. It is relatively difficult to cultivate and improve stress-tolerant traits by traditional breeding techniques, and it is difficult to obtain excellent drought-resistant varieties. With the development of molecular biology technology and the in-depth study of the molecular mechanism of plant stress resistance, the genetic engineering of plant stress resistance has made great progress. It h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/00
CPCC07K14/415C12N15/8273
Inventor 刘庆昌翟红何绍贞赵宁周媛媛
Owner CHINA AGRI UNIV
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