IbAITR5 gene derived from ipomoea batatas, protein encoded by IbAITR5 gene and application of protein
A protein and transgenic plant technology, applied in the IbAITR5 gene and the protein encoded by it and its application fields, can solve the problems of difficult stress tolerance traits and the like
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0099] Embodiment 1, the acquisition of IbAITR5 gene
[0100] 1. Acquisition of cDNA template
[0101] The total RNA of the sweet potato line Xushu 55-2 test-tube seedlings was extracted with a plant total RNA extraction kit, and the first-strand cDNA was reverse-transcribed from the total RNA with a PrimeScriptTM RT reagent Kit with gDNA Eraser kit.
[0102] 2. Using the cDNA obtained in step 1 as a template, design and artificially synthesize primers 3GSP-1 and 3GSP-2, use the RACE method to amplify a 3'-RACE fragment of about 300bp, and combine the 3'-RACE fragment with the cloning vector pMD19- T connection to obtain recombinant plasmid 2. The recombinant plasmid 2 was sequenced to obtain the nucleotide sequence of the 3'-RACE fragment. The primer sequences are as follows:
[0103] 3GSP-1:5'-CGTAAAGCTACCACCGTCCAG-3'
[0104] 3GSP-2:5′-GTGATGTGTGGAGAATGAGCG-3′
[0105] 3. Design and artificially synthesize primers 5GSP-1 and 5GSP-2, use the cDNA obtained in step 1 as a...
Embodiment 2
[0112] Example 2. Application of IbAITR5 protein in improving plant drought resistance.
[0113] 1. Construction of recombinant plasmid pCAMBIA super1300-IbAITR5-GFP
[0114] 1. Artificially synthesized double-stranded DNA molecules shown in positions 1 to 987 from the 5' end of SEQ ID NO.1 in the sequence listing. Using the double-stranded DNA molecule as a template, OE-F-Xba I: 5'-GC TCTAGA ATGGATGGGCGAGGG GG-3' (underlined is the recognition sequence of restriction endonuclease Xba I) and OE-R-Pst I: 5'-AA CTGCAG AAGG ATAGAGCAGCCCTGTA-3' (the underline is the recognition sequence of restriction endonuclease Pst I) is used as a primer for PCR amplification to obtain a double-strand containing restriction endonuclease XbaI at the N-terminus and restriction endonuclease PstI at the C-terminus DNA molecule.
[0115] 2. Digest the vector pCAMBIA super1300-GFP with restriction endonucleases Xba I and Pst I, and recover the vector backbone 1 of about 10783 bp.
[0116] 3. Th...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com