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Application of DCUN1D1 gene to control of vitiligo

A vitiligo and gene technology, applied in the application field of DCUN1D1 gene inhibitor in the preparation of medicine for treating vitiligo

Active Publication Date: 2020-06-05
HANGZHOU THIRD HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The expression level of DCUN1D1 gene in vitiligo skin lesions is significantly higher than that in healthy controls. However, the function of DCUN1D1 gene in vitiligo is still poorly understood, especially the biological function of the gene in keratinocytes and its regulatory mechanism. Research so far unreported

Method used

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  • Application of DCUN1D1 gene to control of vitiligo
  • Application of DCUN1D1 gene to control of vitiligo
  • Application of DCUN1D1 gene to control of vitiligo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] 1. Experimental materials

[0030] 1.1 Experimental animals:

[0031] Clean grade 6-week-old female C57BL / 6 mice were purchased from the Animal Experiment Center of Zhejiang Academy of Medical Sciences. The mice were randomly divided into a PBS group (PBS), a negative control group (Negative control) and an experimental group (AAV-mDCUN1D1), with 4 mice in each group and housed in separate cages.

[0032] 1.2 Feed:

[0033] The animal experiment center of Zhejiang Academy of Medical Sciences provided basic diet for mice.

[0034] 1.3 Reagents:

[0035] Cell-grade PBS was purchased from Gibco, USA, and hydrogen peroxide (H 2 o 2 ) was purchased from Sigma Company of the United States, and the Transwell culture chamber was purchased from Costar Company of the United States. The melanin staining kit was purchased from Hangzhou Fude Biotechnology Co., Ltd. human DCUN1D1 gene primer hDCUN1D1-F: 5'-TGCCTACTGGAACTTAGTGCT-3' and hDCUN1D1-R: 5'-AGTGGTATAGACAGGTCTGTTGG-3'; ...

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Abstract

The invention provides an application of a DCUN1D1 gene to control of vitiligo. Through research, the applicant finds that the DCUN1D1 gene expression of a patient suffering from vitiligo is increased, and experimentation on animals confirms that specific overpression of DCUN1D1 on keratinocyte can enable local facial depigmentation of mice, growth of hair follicles is restrained, the skin thickness is thinned, and chromogenesis in the hair follicles is restrained. The DCUN1D1 gene is promoted to be a new target point of medicines for treating vitiligo, the expression of the DCUN1D1 gene is reduced to promote proliferation of the keratinocyte, reduce apoptosis and promote melanocyte migration, and the application is a new way of treatment with medicines. The application has the beneficialeffects that the new gene target point for treatment of vitiligo is found, a new purpose of a DCUN1D1 gene inhibitor to preparation of medicines for treating facial depigmentation particularly medicines for treatment of vitiligo is provided, and a foundation is provided for screening of new medicines.

Description

[0001] (1) Technical field [0002] The invention relates to the application of DCUN1D1 gene in the prevention and treatment of vitiligo, specifically the application of DCUN1D1 gene in the screening of medicines for treating vitiligo, and the application of DCUN1D1 gene inhibitors in the preparation of medicines for treating vitiligo. [0003] (2) Background technology [0004] Vitiligo is a depigmented skin disease caused by the loss of epidermal melanocytes, which plagues about 1.5% to 2% of the world's population, and causes obvious white spots, seriously affecting the physical and mental health of patients ( A et al, The British journal of dermatology, 2013, 168(1):5-19.). There are many theories on the pathogenesis of vitiligo, and the etiology is unknown, among which cellular immunity is closely related to other pathogenesis (Shah AA and Sinha AA, European journal of dermatology: EJD, 2013, 23(1): 5-132013; Laddha NC et al, Experimental dermatology, 2013 , 22(4):245-25...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K31/7105A61P17/00C12Q1/6883
CPCA61K45/00A61K31/7105A61P17/00C12Q1/6883C12Q2600/158C12Q2600/136
Inventor 关翠萍许爱娥许文
Owner HANGZHOU THIRD HOSPITAL
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