System and method for detecting linalool chiral isomer and application thereof
A technology of linalool and isomers, which is applied in the application field of identifying whether linalool is added to Yunnan bamboo leaf prickly ash oil, can solve the problem of not identifying Yunnan bamboo leaf prickly ash oil to synthesize linalool and the like, and achieves sensitivity High, easy to operate, less sample volume
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[0072] Preparation of natural Yunnan bamboo leaf pepper oil samples
[0073] The bamboo-leaved peppercorns (from Yunnan Zhuoyi Food Co., Ltd.) that have been deseeded are pulverized into 20 mesh powders with a pulverizer (purchased from Tianjin Test Instrument Co., Ltd., model FW80), and then the crushed 1500g bamboo-leafed peppercorns Add supercritical CO 2 Extractor (purchased from Guizhou Aerospace Bird Wujiang Electromechanical Equipment Co., Ltd., customized), the extraction temperature is 50 ° C, the extraction pressure is 25 MPa, CO 2 The flow rate is 70L / h, and the extraction time is 4h. Set the extraction conditions and start the extraction. Collect supercritical CO after extraction is complete 2 The extract produced by separation I and separation II of the extractor was centrifuged to remove water to obtain natural bamboo leaf pepper oil with a yield of 20%.
Embodiment 1
[0078] Add 10 μL of the natural Yunnan bamboo leaf pepper oil prepared above into a 40 mL special bottle for solid-phase microextraction, and put the aged 1-cm long 75 μm Carboxen / polydimethylsiloxane type extraction fiber (trade name: Carboxen / PDMS, black extraction fiber, purchased from Supelco company) was inserted into the headspace of the bottle, extracted at room temperature for 10s, after the extraction was completed, the extraction fiber was taken out, inserted into the inlet of a gas chromatograph (model: Agilent 7890A GC, Agilent Corporation of the United States), and Desorb at 250°C for 5 minutes, and perform gas chromatography analysis. The analysis conditions are that the detector is a hydrogen flame ionization detector, the detector temperature is 280°C, and the chromatographic column used is CHIRALDEX TM B-PM type chiral capillary column (length 50m, inner diameter 250μm, film thickness 0.12μm, purchased from Supelco Company), the initial temperature of the chr...
Embodiment 2
[0080] Add 10 μL of the natural Yunnan bamboo leaf pepper oil prepared above into a 40 mL special bottle for solid-phase microextraction, and the aged 2-cm long 50 / 30 μm DVB / CAR / PDMS type extraction fiber (trade name: DVB / CAR / PDMS, gray extraction fiber, purchased from Supelco company) was inserted into the headspace of the bottle, extracted at room temperature for 10s, after the extraction was completed, the extraction fiber was taken out, and inserted into the inlet of a gas chromatograph (model: Agilent 7890A GC, purchased from Agilent Corporation of the United States) , desorbed at 250°C for 5min, and carried out gas chromatography analysis. The analysis conditions were that the detector was a hydrogen flame ionization detector, the detector temperature was 280°C, and the chromatographic column used was CHIRALDEX TM B-PM type chiral capillary column (length 50m, inner diameter 250μm, film thickness 0.12μm, purchased from Supelco Company), the initial temperature of the ch...
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