Aegilops biuncialis specific molecular marker obtaining method and application thereof

Abstract

The invention discloses an aegilops biuncialis specific molecular marker obtaining method and an application thereof. The method comprises the following steps: extracting genome DNA of aegilops biuncialis to be detected, and then simplifying genome library construction and high-throughput sequencing; comparing an SLAF tag obtained by sequencing after sequence analysis and screening of the SLAF tagof a specific gene with a reference genome sequence of known wheat; and screening sequences of which homology is less than 50% for primer designing; screening specific molecular markers of aegilops biuncialis, further positioning aegilops biuncialis primers obtained by screening to specific genes in aegilops biuncialis by utilizing aegilops tauschii genome donors with specific genes in homologousaegilops tauschii, and performing PCR amplification on the designed primers to obtain the specific molecular markers of aegilops biuncialis. The method can be used for detecting genetic components such as aegilops biuncialis chromosomes and chromosome fragments under a wheat background, can be used for rapidly detecting the wheat-aegilops biuncialis derivative line material on a large scale by simplifying a genome sequencing technology, and is time-saving, labor-saving and low in cost.

Description

technical field [0001] The invention belongs to the technical field of molecular marker screening, and in particular relates to a method for obtaining a specific molecular marker of Alpaca ibex and its application. Background technique [0002] Aegilops L. is the most closely related genus of wheat relatives, plays an important role in wheat evolution, and provides a group of good genes for wheat improvement. Goatwort consists of 11 diploids, 10 tetraploids, and 2 hexaploids (Van Slageren, 1994), including C, D, N, M, S, and U genomes (Mirzaghaderian and Mason 2019). Ae. biuncialis (Ae. biuncialis, 2n = 4x = 28, UUMM) carries excellent genes such as drought resistance, salt tolerance, and disease resistance, but at present, there is little progress in the development and detection of specific molecular markers for Ae. biuncialis. Two molecular markers (GWM44 and GDM61) screened from 108 wheat SSR markers can be used in Goatwort 2M b and 3M b Chromosomal detection; screene...

AI Technical Summary

Problems solved by technology

The use of simplified genome sequencing technology for the development of ibex-specific molecular markers has not yet been seen. This patent proves that this method can be efficiently used to develop ibex-specific molecular markers. Therefore, a simplified genome sequencing technology is needed for large-scale and rapid wheat-ibex Method for obtaining specific molecular markers for the detection of grass-derived materials

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