Aegilops biuncialis specific molecular marker obtaining method and application thereof

A technology of molecular markers and acquisition methods, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to achieve the effect of low cost

Pending Publication Date: 2020-06-12
中国科学院遗传与发育生物学研究所农业资源研究中心
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Problems solved by technology

The use of simplified genome sequencing technology for the development of ibex-specific molecular markers has not yet been seen. This patent proves that this method can be efficiently used to develop ibex-speci...

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  • Aegilops biuncialis specific molecular marker obtaining method and application thereof
  • Aegilops biuncialis specific molecular marker obtaining method and application thereof

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[0022] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. It should be understood that these examples are only for illustrating the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the contents of the present invention, those skilled in the art may make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0023] The present invention comprises the following steps:

[0024] A Simplified genome library construction and high-throughput sequencing after extracting the genomic DNA of Goatwort to be tested;

[0025] B-sequence analysis and screening of SLAF tags for specific genes;

[0026] After the SLAF tags obtained by C sequencing were compared with the known wheat reference genome sequences, th...

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Abstract

The invention discloses an aegilops biuncialis specific molecular marker obtaining method and an application thereof. The method comprises the following steps: extracting genome DNA of aegilops biuncialis to be detected, and then simplifying genome library construction and high-throughput sequencing; comparing an SLAF tag obtained by sequencing after sequence analysis and screening of the SLAF tagof a specific gene with a reference genome sequence of known wheat; and screening sequences of which homology is less than 50% for primer designing; screening specific molecular markers of aegilops biuncialis, further positioning aegilops biuncialis primers obtained by screening to specific genes in aegilops biuncialis by utilizing aegilops tauschii genome donors with specific genes in homologousaegilops tauschii, and performing PCR amplification on the designed primers to obtain the specific molecular markers of aegilops biuncialis. The method can be used for detecting genetic components such as aegilops biuncialis chromosomes and chromosome fragments under a wheat background, can be used for rapidly detecting the wheat-aegilops biuncialis derivative line material on a large scale by simplifying a genome sequencing technology, and is time-saving, labor-saving and low in cost.

Description

technical field [0001] The invention belongs to the technical field of molecular marker screening, and in particular relates to a method for obtaining a specific molecular marker of Alpaca ibex and its application. Background technique [0002] Aegilops L. is the most closely related genus of wheat relatives, plays an important role in wheat evolution, and provides a group of good genes for wheat improvement. Goatwort consists of 11 diploids, 10 tetraploids, and 2 hexaploids (Van Slageren, 1994), including C, D, N, M, S, and U genomes (Mirzaghaderian and Mason 2019). Ae. biuncialis (Ae. biuncialis, 2n = 4x = 28, UUMM) carries excellent genes such as drought resistance, salt tolerance, and disease resistance, but at present, there is little progress in the development and detection of specific molecular markers for Ae. biuncialis. Two molecular markers (GWM44 and GDM61) screened from 108 wheat SSR markers can be used in Goatwort 2M b and 3M b Chromosomal detection; screene...

Claims

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Application Information

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IPC IPC(8): C12Q1/6809C12Q1/6895
CPCC12Q1/6809C12Q1/6895C12Q2600/13C12Q2600/166C12Q2535/122C12Q2537/165C12Q2531/113C12Q2545/113C12Q2565/125
Inventor 宋利强李俊明纪军张玮张娜
Owner 中国科学院遗传与发育生物学研究所农业资源研究中心
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