A kind of pcr-hrm primer and method for distinguishing wild strain and vaccine strain of Riemerella anatipestifer

Abstract

The invention discloses a PCR-HRM primer and a method for quickly distinguishing the wild strain of Riemerella anatipestifer and the GD12 vaccine strain. The present invention establishes for the first time a PCR-HRM detection method that can quickly distinguish the wild strain of Riemerella anatipestifer from the GD12 vaccine strain, which is simple to operate and high-throughput, and significantly shortens the time between the wild strain of Riemerella anatipestifer and the GD12 vaccine strain. The time required for identification and detection of the GD12 vaccine strain is high, the accuracy is high, the specificity is good, and the repeatability is good, which is conducive to popularization and application in clinical practice.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, in particular to a PCR-HRM primer and a method for rapidly identifying the wild strain of Riemerella anatipestifer and the GD12 vaccine strain. Background technique [0002] Riemerella anatipestifer (RA) infection is a contagious disease of ducks, geese, turkeys and wild birds, also known as duck infectious serositis, which mainly affects 1-8 weeks of age (especially 2-8 weeks of age). 3 weeks old) ducklings, goslings and young turkeys, etc. The serotypes of Riemerella anatipestifer are complex, with a total of 21 serotypes, and there is no obvious cross-immune protection between different serotypes, which brings certain difficulties to the prevention and treatment of infectious serositis in ducks. [0003] Due to the abuse of antibiotics in clinical practice, the resistance of bacteria is becoming more and more serious. At present, there are very few drugs that can be used to treat du...

AI Technical Summary

Problems solved by technology

Since the clinical use of live vaccines may bring certain difficulties to the accurate diagnosis of the disease, it is difficult to distinguish whether the clinically isolated Riemerella anatipestifer is a wild strain or a vaccine strain, so it is necessary to establish a vaccine against duck disease. Differentiation and Detection Method of Riemerella Wild Strain and Vaccine Strain

[0004] At present, the detection methods for R. anatipestifer mainly include bacterial isolation and identification, agglutination test, agar diffusion test, ELISA, immunofluorescence technology, ordinary PCR and fluorescent quantitative PCR, etc., but there is no detection method for the wild strain of R. anatipestifer. The key reason for the identification and detection method of vaccine strains is that there are no reliable and effective amplification primers that can be used to distinguish wild strains of Riemerella anatipestifer from vaccine strains.

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